3D Tumorsphere Medium XF

Cancer stem cells (CSCs) have an immense impact on cancer initiation, progression, metastasis, and relapse. Their ability to resist conventional therapies and drive tumor aggressiveness underscores the importance of understanding and targeting CSCs in the fight against cancer.

Download this comprehensive eBook: "Working with cancer stem cells", a complete guide on CSC biology, including an isolation protocol and the latest insights.

In this application note, Promocell Gmbh provides an overview and demonstration of how to use its toolbox of cancer media to build high-quality cancer assay panels with patient-derived organoids (PDOs) to model immunotherapy function. Immuno-oncology is a unique combination of oncology and immunology. By activating or amplifying the body’s natural defenses, immuno-oncology aims to boost the immune system to fight cancer. Targeted antibodies or immunomodulators are well-known immunotherapies; adoptive cell therapy using CAR-T or TCR cells is particularly important, representing a significant breakthrough in cancer therapy with the recent approval of several CAR-T therapies.

The PromoCell Cancer Cell Line Medium XF was designed with the aim of achieving a universal, consistent and xeno-free environment for culturing most of the commonly used human cancer cell lines. Being broadly usable across these common adherently growing cancer cell lines, this new culture medium is a cost-effective solution for ensuring efficient, genuinely standardized routine cultures.

This application note details a successful protocol for extended 3D culture of tumorspheres, from adherently growing human cancer cells, in vitro.

The PromoCell Cancer Stem Cell Medium has been designed to meet requirements for the extended serial 3D tumorshphere culture. Tumorsphere Medium XF supports the most commonly used cancer stem cell lines in tumorsphere/mammosphere culture. In contrast to the current adherent 2D culture of cancer cells, this type of 3D culture selectively exploits inherent biologic features of cancer stem cells such as anoikis resistance and self-renewal. Continuous proliferation is also supported during serial passage of 3D tumorsphere cultures. Thus, this culture system is also applicable for in vitro models of metastasis.

Cancer stem cells permit the culturing of more physiologically relevant 3D tumor microenvironments, and demonstrate the key features of tumors in vivo. This scientific poster illustrates the use of the PromoCell 3D Tumorsphere Medium XF in the successful establishment of extended mammosphere/neurosphere culture of human breast and neural cancer cells.

Tumor growth is sustained by a highly diverse microenvironment, the TME. Its complex cellular interactions need serious consideration during Cancer Research.

The new cancer cell culture toolkit from PromoCell aids the growth of both primary tumors as well as established cells lines, explains Dr. Hagen Wieland. The primary cell culture medium, the core component of the kit, selects for malignancy to yield pure cultures of malignant cells.

From lightning-fast labeling technology to real-time cytometry and high-throughput protein interaction analysis, find out what technologies are making the headlines in life sciences this month