Yarra Ultra-High Efficiency Aqueous GFC/SEC Columns
Starting with 3 µm ultra-pure silica, Yarra particles are densely bonded with a proprietary surface chemistry. Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra GFC columns allow for the highest efficiencies and resolution possible. Yarra columns are offered in three phases (SEC-2000, 3000, and 4000), and are ideal for the separation of small to large proteins, as wel…
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Or lab used it for separation of colostrum whey
We received this column just over a month ago. For the first 100 injections everything was very good. Separation was wonderful. Analysis of a sample took 15 minutes. I thought that I received the best column which you can buy. I used a cleaning solution of 0.1 M Monosodium phosphate pH 3.0 and 20% methanol. A week later I used the column again but separation was awful. I tried to clean it with 0.5 SDS and acetonitrile gradient, but result was the same. Then I connected a 2 year old BioSep-SEC and result was good. So problem was in Yarra column. Phenomenex provide 45 days of return or exchange for their columns. I contact domestic distributor and explain situation. But until now, nothing has changed. Annoyingly that column for more than 1,000 Euro died so quickly.
Review Date: 6 Nov 2014 | Phenomenex Inc
Starting with 3 µm ultra-pure silica, Yarra particles are densely bonded with a proprietary surface chemistry. Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra GFC columns allow for the highest efficiencies and resolution possible.
Yarra columns are offered in three phases (SEC-2000, 3000, and 4000), and are ideal for the separation of small to large proteins, as well as biological therapeutics and biosimilars.
Brochures
Phenomenex Pharmaceutical Analysis Brochure
This brochure provides information on how Phenomenex products can help you achieve resolution, ruggedness and reproducibility in your formulations assays.
LC-MS/MS Environmental Applications
The world is changing and we need to widen our Environmental Scope. Although analytical techniques and established regulated methods are not easily changed; more complex targets, larger lists, and a variety of sample matrices create a daily challenge for environmental chemists. By using the latest technologies and techniques in HPLC/ UHPLC and Sample Preparation, laboratories and chemists are able to develop better approaches to make their work Faster, Cheaper and Easier. Phenomenex is committed to supporting the industry through a comprehensive product portfolio of Sample Preparation, HPLC/UHPLC, and LC-MS, columns and accessories, along with application and method development services to meet industry needs.
Yarra SEC Columns Product Guide
This application note outlines the advantages of using Yarra SEC Columns for protein and peptide separation, including the ability to achieve reliable and reproducible results, large exclusion ranges, extended life time, affordably priced equipment and the offer of a guarantee. The article also includes advice on selecting the best Yarra column for your application and ordering information.
Yarra 1.8μm SEC-X150 Columns - Superior Stability under Harsh Conditions
This application note demonstrates the performance advantages of Yarra 1.8μm SEC-X150 Columns from Phenomenex for the analyses of biomolecules by aqueous size exclusion chromatography (SEC). The switching of mobile phases was used to simulate stress on the silica bed. Faster flow rates served to increase the backpressure to atypical levels and the stoppage of flow after each series of steps mimicked the completion of a series of samples. Column failure was not actually achieved after this study was completed, modeling the stable performance of Yarra SEC-X150 columns over longer lifetimes.
Impressive Yarra 1.8µm SEC-X150 Column Stability After 100 Hours of Harsh Conditions
With HPLC/UHPLC system versatility, a large separation range, Bio-Inert hardware, and high efficiencies, Yarra 1.8 µm SEC-X150 columns bring an amazing set of performance advantages to the analyses of biomolecules by aqueous size exclusion chromatography (SEC). In the development and manufacturing process of the Yarra SEC-X150, extreme care was dedicated to producing stable performance over longer lifetimes than other size exclusion columns on the market have produced. This extended lifetime affords scientists the potential to save significant time and money in addition to the savings already realized by highly affordable list prices. This application note demonstrates a stability investigation using a combination of parameters to create harsh testing conditions. It is important to note that column failure was not actually achieved after this study was completed.
Size Exclusion Chromatography Method Optimization using the European Pharmacopoeia for Insulin Fibrils
Insulin is a peptide hormone which plays a central role in metabolism. In its monomer form, insulin can partially misfold, causing a cascade of non-native cross assembly of beta-pleated sheets or amyloid-like fibrils. A common method for analyzing insulin HMWP is size exclusion chromatography (SEC). However, the method requires a lengthy 35 minute run time which is time prohibitive for any laboratory with many samples to run and data points to generate. This application shows the method transfer of the Ph. Eur. method from a traditional, 10µm hydrophilic silica 300 x 7.8mm column to a high performance, 1.8µm hydrophilic silica 150 x 4.6mm column.
Better Gel Filtration: A New Look at Method Development Parameters Using Yarra™ GFC Columns
An affordable, high efficiency Yarra GFC column was recently introduced and is significantly more efficient than other GFC columns on the market. In addition to higher efficiency, Yarra columns demonstrate significantly higher inertness to ionic interactions versus other GFC columns; however, such chemical characteristics require optimization to operating parameters. Performing method development through mobile phase composition using next-generation Yarra GFC columns will be discussed.
Phenomenex Introduce New Yarra<sup>®</sup> 1.8µm SEC-X300 Columns
UHPLC size exclusion column delivers increased separation of high molecular weight biomolecules
Webinar Highlights: Achieving Greater UHPLC Performance: Three Innovative Surface Chemistries for Reversed Phase, Size Exclusion and Chiral LC Methods
Discover the benefits of new technology and method development strategies for new techniques
