VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette

PCR purification is a critical step in molecular biology workflows, ensuring the removal of primers, nucleotides, and other contaminants that can interfere with downstream applications such as sequencing, cloning, and genotyping. Using magnetic bead-based purification simplifies the process by selectively binding DNA fragments while eliminating unwanted byproducts.

In this application note, INTEGRA demonstrates an efficient and scalable PCR purification protocol using MAGFLO™ PCR beads with the MAG module on VIAFLO 96 and VIAFLO 384. The automated workflow enables high-throughput processing of 96 samples simultaneously, improving consistency and reducing hands-on time.

In this application note, bit.bio highlights how the VIAFLO 96/384 handheld electronic pipette greatly improves ELISA assays. The pipette's 96 channel head enables rapid simultaneous transfer of all samples, enhancing productivity, reproducibility, and saving time.All the steps needed to run the ELISA protocol can be saved on the pipette as a custom program containing all the necessary settings to guide the user through the process via easy to follow prompts.The note details how the VIAFLO 96/384 offers advantages over traditional single or multichannel pipettes, and makes the process faster, less error-prone, and highly reproducible.

Precise handling of liquids is essential to almost all scientific experiments, but in this modern age, manual pipetting alone is not enough to keep up with the requirements of most laboratories. Scientists now demand greater efficiency from their workflows – a trend that has been further intensified by the COVID-19 pandemic – inspiring the development of innovative pipetting solutions to dramatically improve throughput.

Download this application compendium to discover a range of topical resources that detail the effective incorporation of both automated and semi-automated liquid handling technologies across four essential lab techniques: cell culture, cell screening, sample extraction and purification, and PCR.

The eBook explores these core techniques using worked examples spanning a variety of pressing topics, including:

• Automated 3D cell-based assay solutions
• Precise and automated cell seeding
• Genotyping with the ASSIST PLUS pipetting robot
• Transfection-grade plasmid DNA purification
• Rapid purification of DNA from cell and tissue samples
• Automation of RNA extraction and purification
• Genotyping assay and sample screening
• Semi-automated rapid IVD amplification test
• Fully automated PCR assay set-up
• Rapid detection of HPV high-risk strains

Preserving cell viability, avoidance of contamination, high experimental reproducibility, and accuracy are vital for manipulations carried out on cells during media changes, transfers, seeding and passaging. In this application note, INTEGRA Biosciences demonstrates how all of these can be mastered by utilizing the VIAFLO 96 and VIAFLO 384 handheld electronic pipettes.

Precise handling of liquids is essential to almost all scientific experiments, but in this modern age, manual pipetting alone is not enough to keep up with the requirements of most laboratories. Scientists now demand greater efficiency from their workflows - a trend that has been further intensified by the COVID-19 pandemic - inspiring the development of innovative pipetting solutions to dramatically improve throughput.

Download this application compendium to discover a range of topical resources that detail the effective incorporation of both automated and semi-automated liquid handling technologies across four essential lab techniques: cell culture, cell screening, sample extraction and purification, and PCR.

The eBook explores these core techniques using worked examples spanning a variety of pressing topics, including:

• Fully automated 3D cell culture
• Automated seeding of spheroids
• Optimized ELISAs
• High-throughput plasmid DNA purification
• Automated antibody purification
• Simple PCR prep for COVID-19 testing
• Automatic magnetic bead PCR purification

3D models, such as the use of spheroids, are being increasingly utilized for drug discovery. To meet the demands of high-volume drug discovery programs, a high-throughput system is essential. In this application note, INTEGRA Biosciences demonstrates robust quality data for assay development and high-throughput screens generated via an automated method for seeding, dosing, and assaying spheroids. This method uses Corning 1536-well spheroid microplates with the assistance of INTEGRA Biosciences’ liquid handling instruments: the VIAFILL and the VIAFLO 384.

Pipettes should be calibrated annually to maintain their performance. In the interim, it is recommended to perform a routine check to test the functionality of your pipettes in order to be confident with your pipetting results. Follow the guidelines in this poster to properly perform such a routine check.

Liquid handling specialists, INTEGRA have produced a poster that provides informative guidelines to enable any lab that has access to a microbalance, to perform a quick routine check that offers many of the benefits of an annual pipette calibration. The described protocol checks pipette functionality and provides a solid indication of its actual performance. It is not a substitute for an annual calibration performed by a specialized calibration laboratory but does allow you to assure your pipetting quality throughout the year.

Watch this video to learn more about INTEGRA Biosciences' VIAFLO 96/384 channel pipette and find out how it can improve your methods and save you time.

This instructional video shows how INTEGRA Biosciences’ 1536-well plate holder enables easy and fast 1536-well pipetting with the VIAFLO 384 electronic pipette. Learn how a one-off adjustment is required to align the tips exactly with the 1536 grid and how the user interface can be used to set the Z height and choose from a variety of pipetting modes.

Watch this video for a comparison of generic pipette tips, which can suffer from high tip attachment and ejection force, leading to inconsistencies, and INTEGRA GripTips, which provide a precise and consistent tip seal through the unique, integrated system that offers the securest pipette tip connection. Without hammering, the GripTips literally snap onto the pipette’s tip fitting, which prevents tips from falling off.

INTEGRA’s new Row Dilution Plate Holder enables users to perform serial dilutions in rows with VIAFLO 96 and VIAFLO 384. This video gives you a short overview on how to use it.

Watch this video demonstration of a solid phase extraction (SPE) microplate method for reproducible, high throughput isolation of high-quality DNA-free total RNA using the vacuum manifold AURUM from Biorad with INTEGRA’s VIAFLO 384.

Learn about the INTEGRA VIAFLO 384, a new handheld 384-channel electronic pipette that enables fast, precise and easy transfer of 384 samples simultaneously. In this video Jonathan Harkins demonstrates the ease of use of the system and also discusses other key features, such as GripTip technology and interchangeable pipetting heads. Interview filmed by SelectScience at SLAS2013.

In this video Anthony Aglione from Pepsico Global R&D tells SelectScience about his work screening natural product libraries for sugar substitutes in the food and beverage industry. Anthony explains how, since its arrival, the new VIAFLO 384 from INTEGRA has become the workhorse of his laboratory. He also discusses some of the key benefits of the instrument including the GripTip technology, which improves confidence levels when transferring samples. Interview filmed by SelectScience at SLAS2013.

Discover how VIAFLO 96 and VIAFLO 384 handheld electronic pipettes from INTEGRA Biosciences can be used to overcome key challenges associated with cell culture, including contamination, viability, and reproducibility.

Liquid handling solutions for flexible molecular diagnostics workflows

Scientists at Rejuveron use VIAFLO 384 to investigate the ability of drugs to penetrate the blood brain barrier (BBB)

INTEGRA Biosciences offers a range of electronic pipettes, pipetting robots and dispensers tailored to allow gentle pipetting for liquid handling tasks

Discover how INTEGRA pipetting systems can streamline routine pipetting tasks for more efficient and productive workflows in antibiotic development

Handheld electronic pipette used in the study of protein translation

From rapid multiplexed analysis, 3D organoid imaging software, and automated plate processing, discover the top technologies, techniques and best practices for cell-based assay research

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