INTEGRA’s VIAFLO 384 proves indispensable in neurodegeneration research
Scientists at Rejuveron use VIAFLO 384 to investigate the ability of drugs to penetrate the blood brain barrier (BBB)
5 Apr 2022
Scientists at Rejuveron Vascular Therapeutics AG, a company specializing in the development of therapies to tackle neurodegeneration, are using INTEGRA’s VIAFLO 384 handheld electronic pipette to accelerate their research. Combining this convenient benchtop system with endothelial cells cultured in Corning® HTS Transwell® 96 well plates allows the team to investigate the ability of drugs to penetrate the blood-brain barrier (BBB). Vasco Ledebur de Antas de Campos, Head of Discovery Biology at Rejuveron Vascular Therapeutics, explained: “We deposit cells in the Transwells, and incubate them to form a monolayer on the filter membrane in each well. Once we have obtained high confluency – which simulates the BBB – we administer the drug candidates.”
“We use a VIAFLO 384 – with a 96 channel head and 125 µl Sterile Filter GripTips – to both deliver the drugs to the apical side and take samples from the basolateral side of the Transwells. This allows us to check the amount of each drug that was able to permeate the barrier. This approach would be difficult to perform with a standard multichannel pipette, as we are often screening large libraries. In contrast, it’s a simple task for the VIAFLO 384, since you can easily program the system to aspirate from any part of the well.”
“I love working with the VIAFLO 384. The system is easy to set up and optimize and, in addition, it is very flexible. For example, you do not need to load the whole deck, but can use just a single column or row, which means that we can use the system to set up and study dose responses,” Vasco concluded.
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VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette
INTEGRA BiosciencesINTEGRA Biosciences’ VIAFLO 384 increases productivity of sample preparation in microplates and supports 24, 96 and 384 channel pipetting heads. VIAFLO 384 is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. With a choice of ten interchangeable pipetting heads, the unit quickly adapts to any 24, 96, 384, or even 1536 well pipetting application in a volume range of 0.5 μl to 1250 μl. VIAFLO 384 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 384 can be used for sample transfers from reservoir to plate, plate to plate or within plates. VIAFLO 384 Features and Benefits: VIAFLO 384 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. Various pipetting modes are available for increased productivity and application customization.
GripTips for VIAFLO 96/384
INTEGRA BiosciencesWhen working with a 96- or 384-channel system such as the VIAFLO 96/384, it is essential that the pipette tips are reliably attached. Due to the unique design, the GripTip system ensures that the tips will never accidently fall off, even when performing a tip touch on the sidewall of a vessel. Pipetting with multiple channels into a well plate is a precision task. GripTips for VIAFLO 96/384 undergo an additional quality check for tip straightness, guaranteeing accurate pipetting in 96- and 384-well plates. Depending on the tip size, GripTips are packaged in two different tip boxes. Larger volume GripTips (300 µl, 1250 µl) are delivered in boxes of 96 tips. The smaller volume GripTips (12.5 µl, 125 µl) are filled in a space efficient 384-fold grid in XYZ boxes. These boxes house 4 times as many tips and feature the standard SBS-footprint. All tips are available in non-sterile, sterile or filtered sterile configuration. Features and benefits: • GripTips snap onto the tip fitting of the pipetting head, which prevents tips from falling off. • An additional quality check ensures that pipette tips are perfectly straight, enabling precise pipetting in multi well plates. • Filtered GripTips reliably protect the pipetting head against contaminations caused by aerosols.
HTS Transwell®- 96 Reservoir Plate, CellBIND® Treated, Sterile, 10/CS
Corning Life SciencesThe HTS Transwell-96 permeable support has an array of 96-wells with membrane inserts connected by a rigid, robotics-friendly tray that enables all 96 inserts to be handled as a single unit.Choice of either polyester (PET) membrane (0.4 µm, 1.0 µm, and 8.0 µm pore sizes) or polycarbonate (PC) membrane (0.4 µm, 3.0 µm, and 5.0 µm pore sizes) 0.143 cm2 membrane area per well, providing 20% to 50% more surface area for cell growth than other commercially available systemsLarge apical and basolateral access ports allow efficient media sampling and facilitate automated or manual access.Optimized for automation, with multichannel feeder ports, improved gripping surface, and standard bar codesThe reservoir plate allows for simultaneous feeding of 96 wells and comes with a removable media stabilizer to reduce the risk of spills during handlingThe receiver plate isolates each well to enable 96 individual assays.SterileThe HTS Transwell-96 systems (0.4 µm PC, 0.4 µm PET, and 1.0 µm PET) are packaged with the 96-well insert plate in a reservoir plate and includes the 96-well receiver plate with lid.The HTS Transwell-96 plates (3.0 and 5.0 µm PC, 8.0 µm PET) are packaged with the 96-well insert plate in the 96-well receiver plate with lid. Reservoir plates may be purchased separately.