MIRO CANVAS
MIRO CANVAS is a compact digital microfluidics platform for fully automated next generation sequencing (NGS) sample preparation. The revolutionary system drastically simplifies NGS workflows for more walk-away time and higher lab productivity, accelerating genomics discoveries.
MIRO CANVAS
MIRO CANVAS program
MIRO CANCAS cartridge loading
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MIRO CANVAS is a compact microfluidics platform that enables true walk-away automation of complex NGS library prep and hybridization protocols, with only minutes of hands-on time. The affordable and easy-to-use system utilizes an innovative cartridge to minimize reagent usage and accommodate multiple sample preparation steps, including protocols for long-read sequencing technologies, target enrichment and on-demand processing of samples.
- Simple, walk-away automation for on-demand NGS sample preparation
- Minimize costs with up to 75 % reduction in reagent use and only 15 minutes of hands-on time
- Established NGS sample prep protocols for both short-and long-read sequencing platforms
MIRO CANVAS has been optimized to produce high quality results. These include validated protocols for long-read sequencing – such as PacBio sequencing – as well as short-read sequencing applications, including whole genome sequencing (WGS) and target enrichment.
MIRO Technology:
MIRO CANVAS uses digital microfluidics to move droplets and perform operations using electromechanical forces. All protocol steps, including mixing, thermal cycling and magnetic bead clean-ups are performed in the MIRO cartridge and relevant thermal or magnetic zones on the electrode board of the MIRO CANVAS. The technology fully integrates sample preparation steps to provide walkaway operation.
Streamline liquid handling with automation
Cutting-edge research in the life sciences industries is increasingly complex, demanding ever-greater precision. Automated liquid handling is a key driver of increased precision and efficiency in many laboratory processes.
In this second edition SelectScience guide, we investigate several automated liquid handling solutions from INTEGRA.
Download this SelectScience guide to explore:
- Streamlining DNA purification
- Optimizing PCR and sequencing prep
- Cutting-edge genome sequencing automation
- Automation in routine assays
- Simplifying serial dilution
- Product features and benefits
Resource details:
- Document type: SelectScience guide
- Page count: 87
- Read time: 130 mins
- Edition: 2nd
Automated library preparation for a nanopore DNA barcoding protocol on the MIRO CANVAS
Discover the MIRO CANVAS NGS prep system – a digital microfluidics platform that automates next-generation sequencing (NGS) library preparation that can be used in applications for analysis of base modifications, metagenomic and epigenetic studies – or when detecting species of interest. Explore results from the Native Barcoding Kit partially automated on the MIRO CANVAS, which can handle up to eight native barcoded samples or >60 bead purified amplicon samples.
Automating ligation sequencing kit on MIRO CANVAS
Long read sequencing, such as Oxford Nanopore Technologies (ONT), excels in detecting large genomic mutations, covering long repeat regions, and identifying signatures lost due to PCR amplification. ONT's technology allows sequencing of single-stranded DNA and RNA through nanoscale pores, offering portability, low cost, rapid turnaround, and real-time analysis. MIRO CANVAS®, a digital microfluidics platform, automates low-throughput workflows like NGS library preparation, compatible with various reagents. INTEGRA Biosciences outlines results achievable by combining ONT Ligation Sequencing Kit with MIRO CANVAS, producing research-ready libraries for ONT sequencing platforms.
Twist fast hybridization target enrichment for human exome
The Twist Fast Hybridization Target Enrichment Human Exome Hyb Capture for MIRO CANVAS® NGS prep system enriches NGS libraries for human exome sequencing via hybridization capture. Designed for MIRO CANVAS, it's tested with 500 ng of single plex or 1500 ng of 8-plex library pool. INTEGRA Bioscience details materials, equipment, and procedures, including reagents, equipment setup, and step-by-step protocol instructions for preparing the capture pool, washing streptavidin beads, preparing hybridization mix, and loading the cartridge to start the run. Sample recovery and library quantification methods are also covered.
Enabling prep kit on the MIRO CANVAS NGS prep system
The demand for PCR-free next-generation sequencing (NGS) library preparation protocols is increasing to mitigate PCR bias in DNA sequencing pools. The Illumina® DNA PCR-Free Prep kit addresses this need, offering a flexible and easily automatable PCR-free workflow, particularly valuable for sensitive applications like whole genome sequencing (WGS). Leveraging the MIRO CANVAS® digital microfluidics (DMF) platform, this protocol enables custom low-throughput workflow automation for complex NGS library preparation, compatible with a wide range of reagents. INTEGRA Biosciences outlines the outcomes when employing the Illumina DNA PCR-Free Prep kit in a protocol tailored for MIRO CANVAS, with resulting libraries suitable for sequencing on Illumina platforms.
Nanopore genomic DNA by Ligation
The MIRO CANVAS NGS prep system protocol for Nanopore Genomic DNA by Ligation aims to generate research-only libraries for sequencing on the Oxford Nanopore Platform. The protocol details required materials, equipment, and consumables, emphasizing proper handling and storage. INTEGRA Biosciences outlines step-by-step procedures for preparing reagents, loading the MIRO Cartridge, and starting the run, with specific instructions to ensure accurate and efficient execution. Additionally, INTEGRA provides guidance on recovering the sample, finishing the run, and quantifying the final library product, along with a FAQ section addressing common queries and issues encountered during the process.
PacBio SMRTbell Prep 3.0
The PacBio® SMRTbell® Prep 3.0 for MIRO CANVAS® NGS prep system provides PCR-free library preparation for long DNA fragments for use in PacBio sequencing. INTEGRA Biosciences details a list of required materials, equipment, and consumables, along with step-by-step procedures for preparing reagents, loading the MIRO Cartridge, starting the instrument run, and recovering the sample. Additionally, INTEGRA addresses frequently asked questions regarding Dropgloss, pipette tip sizes, residual reagent, bubbles in pipette tips, regurgitation during cartridge loading, and the specific dilution of AMPure® PB beads.
Automating whole genome sequencing library prep on MIRO CANVAS
Long read sequencing is vital for producing high-quality genomes with applications in haplotype phasing, structural variant detection, and de novo assemblies, while PCR-free library preparation eliminates base composition bias. Traditional gel-based size selection methods are limited by DNA input requirements and lack automation. INTEGRA Biosciences highlights the PacBio® SMRTbell® Prep Kit 3.0 which addresses these challenges by offering PCR-free sequencing with fast bead-based size selection, enabling automated library preparation. The MIRO CANVAS®, a digital microfluidics platform, facilitates low-throughput automation for complex NGS protocols, including those using the SMRTbell Prep Kit 3.0, resulting in research-ready libraries compatible with PacBio sequencing systems.
MIRO CANVAS: Fully automated NGS sample prep
In this video, INTEGRA Biosciences introduces MIRO CANVAS, a solution for manual sample preparation challenges in next-generation sequencing (NGS). The system, driven by digital microfluidics, automates intricate NGS library preparation and hybridization protocols, minimizing hands-on time to mere minutes. MIRO CANVAS excels in long-read sequencing, target enrichment, and on-demand sample processing, using electromechanical forces to handle droplets, automate mixing, PCR, and bead cleanup steps. The compact device, equipped with an intuitive interface, operates with a single-use cartridge, designed to significantly reduce reagent usage by up to 75%.
Ready, set, sequence – The evolution and future of NGS
In this guest editorial from INTEGRA Biosciences discover how NGS has transformed genomic research since its initial development, broadening our understanding of genetic variation and gene expression
