QX200™ Droplet Generator (1864002)

This application note demonstrates the incorporation of Bio-Rad’s Droplet Digital™ PCR (ddPCR) system into the library preparation workflow, accurately quantifying and balancing libraries for Ion Torrent sequencing platforms. ddPCR provides highly precise measurements to quantify Ion Torrent libraries without the need for standards.

This comprehensive applications guide presents a variety of uses for the Droplet Digital™ PCR (ddPCR™) from Bio-Rad. Droplet Digital polymerase chain reaction was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications.

This poster presents an exploratory analysis into whether dynamic changes in the levels of plasma EGFR mutations post-osimertinib treatment are associated with clinical outcome. It presents data from the AURA Phase I study in patients with disease progression following prior therapy with an EGFR-TKI and T790M-positive baseline genotyping (in tumor tissue or plasma). It was found that early clearance of plasma EGFR mutations in patients receiving osimertinib therapy appears to be a prognostic marker for improved median progression-free survival of patients.

Detection and quantitation of specific mutations in circulating plasma holds promise for earlier and less invasive diagnosis of disease. Learn how a simple droplet digital PCR strategy can detect somatic mutations with high selectivity and sensitivity.

Learn how liquid biopsies are replacing solid biopsies and are increasing the speed of cancer diagnosis, improving outcomes for patients. Also, find out how liquid biopsy can be used to quantify and monitor mutation, enabling rapid decision-making and treatment plans.

Professor of Genetics, Diptasri Mandal and graduate student, Kirsten Wood, of the Department of Genetics at Louisiana State University Health Sciences Center, explain how custom digital PCR assays are facilitating their research into hereditary biomarkers of prostate cancer.

Scientists at the New York University School of Medicine explain how digital PCR has enabled the identification of novel biomarkers of cancer from liquid biopsies. Digital PCR assists them in detecting and counting low amounts of cell-free DNA being investigated as a promising new biomarker for metastatic disease. Watch the video to learn more.

In this webinar Dr Dawne Shelton discusses how Droplet Digital PCR (ddPCR) can be used to develop personalized cancer care by identifying cancer subtypes and optimizing drug treatment plans. Dr Shelton summarizes work covering the tracking of cell-free tumor DNA during patient progression of disease and treatment monitoring, early detection of tumor cfDNA in blood, detection of copy number amplifications of common genes like MYC, HER2, and EGFR, as well as the detection of KRAS mutations using a multiplex screening strategy.

In this video Bio-Rad Laboratories showcase the QX100™ Droplet Digital™ PCR (ddPCR™) system, which provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The Droplet Digital PCR system is the third generation of PCR technology and provides a revolutionary approach to target DNA quantification.

In this video, researchers Yiping Cao and John Griffith of the Southern California Coastal Water Research Project (SCCWRP) describe their work using Bio-Rad's Droplet Digital PCR (ddPCR™) platform to determine variations in water quality from coastal watersheds to the ocean. Environmental monitoring for genetic targets such as pathogens, invasive species, or other biological activity requires the ability to detect small target concentrations within complex, non-uniform samples.

Discover how Bio-Rad’s Automated QX200™ Droplet Generator can generate a full 96 well plate from your samples in less than 30 mins, reducing hands-on time, simplifying your workflow, and increasing data reproducibility. Also, learn how the QX200™ Droplet Digital™ PCR System now supports EvaGreen chemistry applications, offering users more flexibility. Interview filmed by SelectScience at AACR 2014.

Guest Editor, Randall Evans, Ph.D of Biodesix, discusses the recent success of a clinical trial of a diagnostic blood test

The first digital PCR instrument with the CE IVD mark

Advances in biomarker research using Droplet Digital™ PCR