NucleoCounter® NC-3000™ advanced cell analyzer
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay.
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Easy to use equipment for routine cell count
Research
Very nice equipment, easy to use, fast to measure 8 samples in a go.
Review Date: 10 Oct 2024 | ChemoMetec
An indispensable device in the laboratory!
general laboratory work
We have been using the NC-3000 (Chemometec) for several years and use it daily in the lab. The system is very versatile as it can not only perform cell counts (including cell cycle and Annexin V assays). In many projects, we work with external partners who need the analyses, for example, for certification of their devices. This is easily possible with the NC3000, which is compatible with 21 CFR Part 11/GMP guidelines.
Review Date: 24 Oct 2023 | ChemoMetec
Easy to use, fast results, consistency in data produced
Day to day cell culture counting; cell numbers & viability
Pros: Fast & easy cell counting, good assay/instrument for keeping track of suspension cell culture growth (when to split culture and numbers to seed for transfection (VPC2.0 from Thermo-Fisher)) We have standardized when to dilute cell culture (expected cell dens above 2E6/ml always dill 1:10; 50µl ad 500µl) Cons: Sometimes (rare events) counting fails due to camera not able to take pictures? Solution is to close program and restart (but you often "loose" a few Via1 cassettes) Regarding software; would be nice to have the time in data set
Review Date: 24 May 2023 | ChemoMetec
One of our major cell culture laboratory device!
Cell culture
Usefull in routine studies (including GLP grade), easy, fast, accurate.
Review Date: 5 Oct 2021 | ChemoMetec
Simplifies cell counts, cell cycle analysis and annexin-V assay. Fantastic technology.
Cell counts, cell cycle analysis and annexin-V
Cell counting represents the most basic analytical technique in biotechnology as every single step of a bioprocess relies on knowing the exact cell concentration at the start and at the end. We have been using the NC-3000 (Chemometec) for over 4 years and we are huge fans of it. The system not only allows you to perform cell counts (on suspension cells but also with cells attached to microcarriers) but also cell cycle and annexin-V assays which would normally be performed with flow cytometry. The system stands out in the market as many competitors use purely imaging methods to determine cell counts, viability, and diameter. The NC-3000 (and others from the same company I imagine) use a laser-based system and multiple dyes to detect cell concentration. Our internal validation suggests that the NC-3000 is more precise than alternatives such as manual-based cell counts but also very well known competitors. This would be the first piece of equipment I would purchase when setting up a new lab.
Review Date: 27 Mar 2021 | ChemoMetec
Easy-to-use and convenient.
Cell counting and transduction efficiency calculations
The NC-3000 is a robust and easy-to-use cell imaging system, which we use for counting hematopoietic cells and for estimating transduction efficiency. There is a good range of pre-designed assays on the instrument, and the Protocol Adaptation Wizard allows you to easily adapt protocols for your own applications. The 'flow cytometry-like' dot plots are really useful and you can adjust gates accordingly. We mainly use the Via1 cassettes as we don't count numerous samples at the same time, but we are confident in the reproducibility of our data.
Review Date: 16 Mar 2021 | ChemoMetec
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay. With the FlexiCyte™ license, you can create protocols choosing light source, emission filters and detailed settings. The accompanying NucleoView™ software can be used for data analysis of the results and has unlimited licenses.
The NucleoCounter® NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility, and it is calibration-free. The NucleoCounter® NC-3000™ is ideal for the regulated QC, production and even PD and R&D departments. Instrument and method verification tools and 21 CFR Part 11-ready software ensures that the adheres to the requirements and demands of the pharmaceutical and biotech industries. Standardized pdf-reports, user control and registration of audit trails allow for documentation of all procedures. Combined with high precision and limited user influence this makes the NucleoCounter® NC-3000™ a one-of-a-kind cytometer.
Our Field Application Scientists (FASs) provide training, service visits, installation and user guidance on-site or online to ensure you get the best start to working with the NucleoCounter® NC-3000™.
Key features:
- No rinsing or clogging
- Easy to operate – no warm-up or calibration
- CFR 21 Part 11 ready
- Automated PDF reports
- Unlimited software licenses and free software updates
- Excellent support by field application specialists
- High-speed cell counting and advanced cell analysis
- Visual inspection of cells – plot to image
- Images are saved automatically and can be re-analyzed later
- Low sample volume
- Build your own assays with up to 4 analytical channels
- Export data to FCS Express, FlowJo, etc.
Quantitative assessment of apoptotic events using image and flow cytometry
Cell death by apoptosis is a complex, tightly regulated process in which a cell orchestrates its own destruction in response to a specific internal or external stimuli. Dysregulation of apoptosis can lead to various physical disorders such as cancer and autoimmunity.
In this scientific poster, ChemoMetec demonstrates how the NucleoCounter® NC-3000™ image cytometer can be used to quantify different events in the apoptosis process.
A comparison of DNA quantitation by image and flow cytometry
In this scientific poster, researchers demonstrate accurate and precise determination of cell cycle stages compared to flow cytometric analyses, by utilizing the NucleoCounter® NC-3000™ from ChemoMetec.
NucleoCounter: Publications review
In 2024, ChemoMetec was featured in over 150 publications spanning various research areas, including cell biology, neurology, gene therapy, oncology, stem cells, bioprocessing, and cell-based immunotherapy.
In this eBook, ChemoMetec presents 30 selected publications that show case the versatile usage of its NucleoCounter® NC-200™, NC-202™, and NC-3000™ instruments across multiple disciplines.
A simple and rapid method for DNA content measurement
DNA content can be determined using fluorescent DNA-selective strains that exhibit emission signals proportional to DNA mass. Cellular fluorescence is measured by flow, image, or laser scanning cytometry.
In this scientific poster, ChemoMetec presents a simple, rapid and robust method for analyzing and quantifying the DNA content of cells, utilizing the NucleoCounter® NC-3000™ image cytometer.
A novel rapid apoptosis assay based on thiol redox status
Apoptosis is a highly regulated process characterized by a number of distinct biochemical and morphological changes.
In this scientific poster, ChemoMetec presents an assay for determining the level and distribution of thiols in a cell population, utilizing the NucleoCounter® NC-3000™ image cytometer.
A decrease in cellular thiols caused by drug-induced apoptosis is easily quantified using the novel vitality assay
In this scientific poster, ChemoMetec presents an assay for determining the level and distribution of thiols in a cell population, utilizing the NucleoCounter® NC-3000™ image cytometer. Additionally demonstrating that a lowered level of cellular thiols correlates with known apoptosis assays.
NucleoCounter® NC-3000™: Two-step cell cycle analysis
In this application note, Chemometec describes how the NucleoCounter® NC-3000™ system enables the user to rapidly quantify DNA content of mammalian cells allowing determination of G0/G1, S and G2/M cell cycle phases.
Manual vs. automated cell counting: Overcoming four major sources of error in manual cell counting
In this application note, Chemometec explains how to overcome the four main challenges in cell counting: human perception of what defines a cell; volume, dilution, and pipetting errors; viability determination; counting enough events.
Advanced cell analyses of mammalian cells using the NucleoCounter NC-3000'
Cell death can occur by two distinct mechanisms, necrosis or apoptosis. Necrosis occurs when cells are exposed to harsh physical or chemical stress (e.g., hypothermia, hypoxia) while apoptosis is a tightly regulated biochemical process by which cells are eliminated and where the cell is an active participant in its own termination (“cellular suicide”). This application note demonstrates how to use NucleoCounter® NC-3000™ to detect cells with caspase activity using the Fluorochrome-labelled Inhibitor of Caspases Assay.
NucleoCounter® NC-3000™: Cell Count & Viability of Mammalian Cells using the Via1-Cassette™
The NucleoCounter® NC-3000™ system enables the user to perform automated cell counting and analyses of a broad range of mammalian cells. This application note demonstrates how the Via1-Cassette™ used together with the NucleoCounter® NC-3000™ facilitates determination of viability and concentration of cell suspensions by measuring cell counts (total and non-viable) per volume in one simple step.
The meticulous researcher’s guide to validating a cell counting method
Like any methodology used in the laboratory, it’s important to validate and demonstrate that the cell counting method of choice is precise. But finding the correct methodology for testing this can be challenging, depending on the process and the sample type. In this video by Chemometec, find out how to choose an effective cell counting method for your cell counting research.
NC-3000 cytometry analysis in the cell lab
The NucleoCounter® NC-3000™ is an advanced cell analyzer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells.
Pre-defined assays include a unique 5-minute cell cycle assay and a selection of apoptosis assays.
The NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility and is maintenance and calibration-free.
ChemoMetec achieves gold status with a new Seal of Quality award
ChemoMetec's family of cell counters are being recognized for receiving consistently outstanding customer feedback, again
ChemoMetec achieves silver status, with a new Seal of Quality award
These cell counters are being recognized for receiving consistently outstanding customer feedback, again
