Advanced cell analyses of mammalian cells using the NucleoCounter NC-3000'

Cell death can occur by two distinct mechanisms, necrosis or apoptosis. Necrosis occurs when cells are exposed to harsh physical or chemical stress (e.g., hypothermia, hypoxia) while apoptosis is a tightly regulated biochemical process by which cells are eliminated and where the cell is an active participant in its own termination (“cellular suicide”). This application note demonstrates how to use NucleoCounter^® NC-3000™ to detect cells with caspase activity using the Fluorochrome-labelled Inhibitor of Caspases Assay.