KAPA HiFi PCR Kits
KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…
Achieve the highest fidelity
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Kapa - Worth trying it once for your complex custom PCR projects
Molecular Biology - PCR
It's a novel B-family DNA polymerase, specifically engineered to increased it's affinity for DNA without accessing accessory proteins or binding domains. We used to have many custom projects with different gene targets of various sizes and multiple gene panels. We had to switch from 5-6 different polymerase to this Kappa enzyme. Now for most of our projects Kappa is the first and only choice The results are amazing both on gel (quality) and quantity (ND/qubit). The concentration was good enough to use for other downstream applications. You don't need to do many trials while using this polymerase enzyme. It's quite stable and easy to set-up any PCR evading complexity of PCR program. It may be a bit costly but the price is worth for the value of product
Review Date: 4 Mar 2023 | Kapa Biosystems, Inc.
Good product, gets the job done.
DNA Amplification (PCR)
We go through several rounds of PCR in the lab and constantly have KAPA on hand. KAPA HotStart ReadyMix has worked great every time. Our Customer service reps, are great and always very helpful. The quality of the product and the service are both good.
Review Date: 27 Feb 2019 | Kapa Biosystems, Inc.
We rely on it every day and have produced >300,000 NGS samples with it. It's great!
NGS Library Prep
We use the Kapa HiFi PCR mastermix for high-throughput NGS library prep. We prepare 3,000 nextera-based NGS libraries per day, and use Kapa HiFi PCR mastermix for every prep. We have found HiFi to give us the best and most reproducible results with our automated workflow. We have found that kapa hifi is more forgiving in terms of allowing us to run more cycles of pcr without drastically increasing our duplication rate in the NGS data. This allows us to rescue samples on the lower quality or quantity side. Great product!
Review Date: 31 Oct 2018 | Kapa Biosystems, Inc.
KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends.
KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.
Features:
Achieve the highest fidelity
- Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
- The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion
Improve performance on GC- and AT-rich templates
- Achieve higher success rates across targets up to 84% GC content
- Higher yields when amplifying AT-rich templates
Amplify longer targets with greater sensitivity
- High-fidelity PCR of long and complex genomic templates
- Improved sensitivity
- High speed allows significantly shorter reaction times for long range PCR
Applications:
- High-fidelity PCR
- Amplification of DNA fragments for cloning and protein expression or genomic characterization
- Site-directed mutagenesis
- Next-generation sequencing library amplification
Robust Routine Amplification of Long DNA Fragments Using KAPA Biosystems LongRange HotStart and HiFi HotStart
This application note describes two solutions for genetic testing of long amplicons from KAPA Biosystems: LongRange HotStart and HiFi HotStart PCR kits. Some genetic and pharmacogenetic tests require the routine amplification of long DNA fragments from complex genomic targets. Assays targeting fragments >3 kb often require careful optimization of reaction parameters, such as Mg2+, primer and template concentrations and cycling parameters. KAPA LongRange HotStart offers robust amplification in single and multiplex assays, whereas KAPA HiFi HotStart is capable of amplifying human genomic targets >10 kb with an error rate up to 50 times lower than long-range enzyme blends.
Site-directed Mutagenesis Using DNA Polymerase
Site-directed mutagenesis is a powerful tool for protein engineering and the study of protein structure and function. Numerous methods of site-directed mutagenesis that exploit the primer extension technique have been developed. These methods generate heteroduplex species containing one mutated and one non-mutated strand; subsequent replication and segregation of these molecules inevitably results in a population heavily contaminated with wild-type DNA. Various methods that circumvent the replication of non-mutated DNA or that separate the two species have been developed, but these methods tend to be more difficult and lengthy. This application note describes a fast, simple, and efficient system for the introduction of specific mutations, insertions and/or deletions into DNA cloned in a double-stranded plasmid.
Improve your NGS and Enable Novel Applications with Engineered DNA Polymerases from Kapa Biosystems
Using a number of case studies, this poster illustrates that engineered DNA polymerases are capable of providing better sequence coverage and are enabling novel and challenging NGS applications. The vast majority of PCR applications utilize a small number of wild-type DNA polymerases; improvements to existing applications and the development of novel PCR applications have primarily been realized via innovative changes to all of the reaction components except the polymerases themselves. Kapa Biosystems have engineered a new generation of improved DNA polymerases such as the KAPA HiFi. These are capable of robust amplification of diverse targets spanning a wide range of sizes and GC-content, and KAPA HiFi has demonstrated production of high yields with remarkably little amplification bias when used for NGS library amplification.
Co-optimization of Probes and Polymerases to Drive the Evolution of Targeted Sequencing
This poster demonstrates the capabilities of targeted sequence enrichment using the KAPA HTP Library Preparation Kit and KAPA HiFi HotStart DNA Polymerase. Inefficiencies in library construction and automation-unfriendly workflows have previously meant low throughput and poor performance. By combining enzymes specifically tailored for high performance NGS applications with innovative oligonucleotide probe design, improved library construction and targeted enrichment can be achieved.
