Robust Routine Amplification of Long DNA Fragments Using KAPA Biosystems LongRange HotStart and HiFi HotStart

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.   Features: Amplify long targets with high sensitivity Amplification of targets with high sensitivity and specificity up to 15 kb High yields with low input DNA Outperforms with complex targets Exhibits high yield, greater sensitivity and specificity where competitor long-range systems fail   Applications: Amplification of long targets and/or low amounts Standard short- and mid-range PCR T-A cloning Targeted sample enrichment for next-generation DNA sequencing

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.   Features: Achieve the highest fidelity Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion Improve performance on GC- and AT-rich templates Achieve higher success rates across targets up to 84% GC content Higher yields when amplifying AT-rich templates Amplify longer targets with greater sensitivity  High-fidelity PCR of long and complex genomic templates Improved sensitivity High speed allows significantly shorter reaction times for long range PCR   Applications: High-fidelity PCR Amplification of DNA fragments for cloning and protein expression or genomic characterization Site-directed mutagenesis Next-generation sequencing library amplification