Improve your NGS and Enable Novel Applications with Engineered DNA Polymerases from Kapa Biosystems

KAPA HiFi DNA Polymerase provides high fidelity next-generation sequencing library amplification with the highest yield, lowest bias, and most uniform sequence coverage available. These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which typically produces low concentrations of AT-rich DNA. KAPA HiFi HotStart Uracil+ ReadyMix (2X) is a ready-to-use cocktail containing all components required for PCR, except primers and template.   Features: Higher yields and reduced size bias More uniform sequence coverage Improved representation of AT-rich sequences   Applications: Methylation analysis – amplification of bisulfite-converted DNA for sequencing Amplification of damaged DNA samples Prevention of false-positive results due to carryover amplicon contamination

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.   Features: Achieve the highest fidelity Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion Improve performance on GC- and AT-rich templates Achieve higher success rates across targets up to 84% GC content Higher yields when amplifying AT-rich templates Amplify longer targets with greater sensitivity  High-fidelity PCR of long and complex genomic templates Improved sensitivity High speed allows significantly shorter reaction times for long range PCR   Applications: High-fidelity PCR Amplification of DNA fragments for cloning and protein expression or genomic characterization Site-directed mutagenesis Next-generation sequencing library amplification

KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing. Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particularly from low input and difficult samples such as FFPE and ChIP DNA. Features: Achieve greater molecular complexity Higher yields of adapter-ligated library translates to higher molecular complexity Fewer cycles of amplification are needed, which results in lower PCR duplication rates PCR-free workflows possible from inputs ≥100 ng Reduce amplification bias and improve coverage KAPA HiFi reduces PCR bias Improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions Create high-quality libraries from challenging samples High-quality whole exome sequencing from libraries prepared using as little as 10 ng human genomic DNA High success rates with 250 ng FFPE or less Routine library construction from ≥100 pg ChIP DNA Automate with pre-validated scripts PerkinElmer Sciclone NGS and NGSx Beckman Coulter Biomek FXp and FX dual-pod Agilent Technologies NGS Workstation Option B Eppendorf epMotion 5075 TMX   Applications: Whole Genome Sequencing ChIP Sequencing Whole Exome Sequencing Amplicon Sequencing Targeted Sequencing (Capture) Methyl Sequencing