KAPA DNA Library Preparation Kits for Illumina
KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing. Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particu…
Key sequencing metrics
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KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing.
Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particularly from low input and difficult samples such as FFPE and ChIP DNA.
Features:
Achieve greater molecular complexity
- Higher yields of adapter-ligated library translates to higher molecular complexity
- Fewer cycles of amplification are needed, which results in lower PCR duplication rates
- PCR-free workflows possible from inputs ≥100 ng
Reduce amplification bias and improve coverage
- KAPA HiFi reduces PCR bias
- Improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions
Create high-quality libraries from challenging samples
- High-quality whole exome sequencing from libraries prepared using as little as 10 ng human genomic DNA
- High success rates with 250 ng FFPE or less
- Routine library construction from ≥100 pg ChIP DNA
Automate with pre-validated scripts
- PerkinElmer Sciclone NGS and NGSx
- Beckman Coulter Biomek FXp and FX dual-pod
- Agilent Technologies NGS Workstation Option B
- Eppendorf epMotion 5075 TMX
Applications:
- Whole Genome Sequencing
- ChIP Sequencing
- Whole Exome Sequencing
- Amplicon Sequencing
- Targeted Sequencing (Capture)
- Methyl Sequencing
Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the epMotion<sup>®</sup> 5075 TMX
This application note describes the successful automation of Illumina ChIP-Seq library preparation on the Eppendorf epMotion® 5075 TMX, using the KAPA High-Throughput Library Preparation Kit from Kapa Biosystems. ChIP-Seq library preparation can be a challenging procedure to automate because of its low ChIP DNA input. The combined advantages of the Eppendorf epMotion® 5075 TMX and the KAPA High-Throughput Library Preparation Kit offer an industry-leading solution for standard and low-input library preparation, particularly for ChIP-Seq.
Improve your NGS and Enable Novel Applications with Engineered DNA Polymerases from Kapa Biosystems
Using a number of case studies, this poster illustrates that engineered DNA polymerases are capable of providing better sequence coverage and are enabling novel and challenging NGS applications. The vast majority of PCR applications utilize a small number of wild-type DNA polymerases; improvements to existing applications and the development of novel PCR applications have primarily been realized via innovative changes to all of the reaction components except the polymerases themselves. Kapa Biosystems have engineered a new generation of improved DNA polymerases such as the KAPA HiFi. These are capable of robust amplification of diverse targets spanning a wide range of sizes and GC-content, and KAPA HiFi has demonstrated production of high yields with remarkably little amplification bias when used for NGS library amplification.
Application of NGS in the Diagnostic Lab on Archival FFPE Tissues
Hosted by Kapa Biosystems at AMP2015, Dr. Brian Walker of the Royal Marsden Hospital presents at a work shop entitled "Application of NGS in the diagnostic lab on archival FFPE tissues".
