Freedom EVO® NGS Workstation
The Freedom EVO NGS workstation combines Tecan’s expertise in laboratory automation with sequencing-verified protocols to offer hassle-free NGS sample prep at the touch of a button. NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor intensive and time consuming. The Freedom EVO NGS workstation has been developed to offer robust and reliable automation of library preparatio…
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The Freedom EVO NGS workstation combines Tecan’s expertise in laboratory automation with sequencing-verified protocols to offer hassle-free NGS sample prep at the touch of a button.
NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor intensive and time consuming. The Freedom EVO NGS workstation has been developed to offer robust and reliable automation of library preparation, quantification, qPCR set-up, normalization, pooling and capture. Offering user-friendly, walkaway automation at the touch of a button, it eliminates the need for extensive manual processing, helping to simplify day-to-day activities and ensure high quality results.
Freedom EVO NGS workstation includes all the modules required for precise set-up of NGS sequencing libraries and, with one-touch method selection and step-by-step user instructions via Tecan’s intuitive TouchTools™ graphical interface, it reduces hands-on time and increases productivity – even for inexperienced users. The system’s key benefits include:
- Precise pipetting – from 1,000 µl down to 0.5 µl using eight pipetting channels and innovative air displacement pipetting technology
- Integrated temperature control – to keep reagents cool and provide optimal enzymatic conditions
- Efficient bead clean-up – using a 96-position magnetic plate separator (Alpaqua 96S Super Magnet Plate)
- Walkaway processing – with on-board consumables storage for long unattended runs
- Straightforward operation – with step-by-step set-up instructions
To further streamline processing, Tecan has worked with leading industry partners to supply ready-to-run protocols for a number of popular NGS technologies. Protocols for Illumina’s TruSeq™ Nano DNA, TruSeq Stranded mRNA* and Nextera™ Rapid Capture Exome* chemistries have been developed in collaboration with Illumina and the High-Throughput Sequencing Facility, University of North Carolina at Chapel Hill, with thorough testing and sequencing data analysis to ensure high quality results. In addition, the system is supplied with protocols covering the Ion Torrent™ library preparation workflow for Ion AmpliSeq™ panels, developed and supported by Thermo Fisher Scientific.
*Illumina-qualified protocol.
Automated High Yield Extraction of gDNA from Plant Samples
This application note demonstrates the potential for high yield gDNA extraction using the MACHEREY-NAGEL NucleoSpin® Plant II Midi kit on a Freedom EVO® platform. Plant research is often geared towards crop improvement, and focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. It is often necessary to sequence the genome, creating the need for purification of high quantities of gDNA. DNA extraction from plant material is therefore an integral step in plant research.
Fast and Efficient PCR Clean-Up with the NucleoSpin<sup>®</sup> 96 PCR Clean-Up Kit on a Freedom EVO<sup>®</sup> Platform
This application note demonstrates how MACHEREY-NAGEL’s NucleoSpin® 96 PCR Clean-up kit and Tecan’s Freedom EVO® platform can be used in combination to provide a flexible automated solution for the purification of PCR products. PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantageous to perform in a high throughput format.
Automated set-up of Ion AmpliSeq™ Libraries on the Freedom EVO® NGS workstation
This application note demonstrates how automated library preparation can be performed using the Freedom EVO NGS workstation on up to 96 samples in less than 6 hours, providing libraries that are ready for downstream template preparation and amplification. The Ion AmpliSeq library preparation workflow is a simple, addition-only protocol that is suitable for high throughput automation.
Automated NGS Sample Preparation with the Freedom EVO NGS Workstation
Automation of NGS sample preparation on the Tecan Freedom EVO NGS workstation increases throughput, reproducibility and guides users through each step of protocol selection and worktable set-up with the TouchTools operator interface. Tecan is developing and optimizing automated NGS sample preparation protocols in collaboration with key research centers, partners including Illumina® and other NGS sample preparation kit vendors to qualify and test the protocols to ensure that they work in your laboratory.
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