Automated NGS Sample Preparation with the Freedom EVO NGS Workstation
23 Oct 2014
Automation of NGS sample preparation on the Tecan Freedom EVO NGS workstation increases throughput, reproducibility and guides users through each step of protocol selection and worktable set-up with the TouchTools operator interface. Tecan is developing and optimizing automated NGS sample preparation protocols in collaboration with key research centers, partners including Illumina® and other NGS sample preparation kit vendors to qualify and test the protocols to ensure that they work in your laboratory.
About the company
Tecan
Tecan is a leading global provider of laboratory instruments and solutions in biopharmaceuticals, forensics, and clinical diagnostics. The company specializes in the development, production and distribution of instruments and automated workflow solutions for laboratories in the life sciences sector. Its clients include pharmaceutical and biotechnology companies, university research departments, forensic and diagnostic laboratories. As an original equipment manufacturer, Tecan is also a leader in developing and manufacturing OEM instruments and components that are then distributed by partner companies. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both Europe and North America and maintains a sales and service network in 52 countries.
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Freedom EVO® NGS Workstation
TecanThe Freedom EVO NGS workstation combines Tecan’s expertise in laboratory automation with sequencing-verified protocols to offer hassle-free NGS sample prep at the touch of a button. NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor intensive and time consuming. The Freedom EVO NGS workstation has been developed to offer robust and reliable automation of library preparation, quantification, qPCR set-up, normalization, pooling and capture. Offering user-friendly, walkaway automation at the touch of a button, it eliminates the need for extensive manual processing, helping to simplify day-to-day activities and ensure high quality results. Freedom EVO NGS workstation includes all the modules required for precise set-up of NGS sequencing libraries and, with one-touch method selection and step-by-step user instructions via Tecan’s intuitive TouchTools™ graphical interface, it reduces hands-on time and increases productivity – even for inexperienced users. The system’s key benefits include: Precise pipetting – from 1,000 µl down to 0.5 µl using eight pipetting channels and innovative air displacement pipetting technology Integrated temperature control – to keep reagents cool and provide optimal enzymatic conditions Efficient bead clean-up – using a 96-position magnetic plate separator (Alpaqua 96S Super Magnet Plate) Walkaway processing – with on-board consumables storage for long unattended runs Straightforward operation – with step-by-step set-up instructions To further streamline processing, Tecan has worked with leading industry partners to supply ready-to-run protocols for a number of popular NGS technologies. Protocols for Illumina’s TruSeq™ Nano DNA, TruSeq Stranded mRNA* and Nextera™ Rapid Capture Exome* chemistries have been developed in collaboration with Illumina and the High-Throughput Sequencing Facility, University of North Carolina at Chapel Hill, with thorough testing and sequencing data analysis to ensure high quality results. In addition, the system is supplied with protocols covering the Ion Torrent™ library preparation workflow for Ion AmpliSeq™ panels, developed and supported by Thermo Fisher Scientific. *Illumina-qualified protocol.
