Chromium Single Cell Gene Expression Flex
GEM-X Flex Gene Expression with Feature Barcode technology Single cell RNA sequencing is increasingly being used to profile larger numbers of patient cohorts or different perturbations, making an efficient and scalable workflow paramount.
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Fixation with paraformaldehyde (PFA) allows samples to be collected, shipped to a central location, and analyzed without sacrificing data quality, creating new possibilities for sample accessibility, throughput, and batched analysis.
Multiomic capabilities allow for more thorough characterization of cell populations with high throughput capture of cell surface and intracellular proteins alongside gene expression.
Advanced chemistry also brings single cell transcriptomic profiling to FFPE tissue. Profile up to 2.56 million fixed single cells at once with a scalable workflow that can be run on your schedule, while accommodating the needs of translational and clinical labs where fragile samples or time constraints would otherwise preclude analysis.
- Fix fresh samples at collection to lock in biological states and preserve fragile cells
- Make the most of every sample with reduced cell input recommendations and up to 80% sample prep recovery
- Scale seamlessly with increased throughput capabilities to profile up to 2.56M cells per chip
- Reduce experimental variability and increase efficiency by batching and multiplexing samples
- Get large-scale and highly sensitive gene expression plus protein measurements
- Expand single cell possibilities with access to archived samples
Resolving tumor microenvironment heterogeneity at the single cell level from fixed samples
The tumor microenvironment (TME) has been recognized as a key factor in determining therapeutic responses across various cancer types, yet efforts to explore the full depth and complexity of the TME have been hindered by a lack of adequate tools. Bulk RNA sequencing, flow cytometry, and immunohistochemistry have all led to landmark discoveries in cancer research. However, they have also fallen short due to their lack of resolution, scale, and sample accessibility.
In this webinar, 10x Genomics will present the Single Cell Gene Expression Flex assay, which enables high-throughput RNA sequencing at the single cell level from fixed samples. By introducing simple and ubiquitous fixation conditions, researchers can perform routine whole transcriptome profiling of ~1M single cells across as many as 128 unique samples, while removing supply chain and time constraints from experimental designs. This advanced chemistry allows scientists to investigate the full complexity of the TME and uncover novel insights into cancer biology.
Key learning objectives
- Discover how single-cell resolution can reveal rare cell populations and the full extent of TME heterogeneity
- Understand how high-throughput single-cell RNA sequencing (scRNA-seq) can help identify novel biomarkers and inform therapeutic development
- Learn how the Gene Expression Flex assay expands access with the ability to store and transport samples without losing data quality
Who should attend?
- Researchers
- Principal investigators
- Professors
- Scientists
- Post-Doctoral
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
