BluePippin Size Selection System
The BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples. The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries, or upstream of p…
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The BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples.
The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries, or upstream of protein mass-spec.
For DNA applications, the BluePippin features pulsed-field electrophoresis to collect high molecular weight targets, and can collect fragments (depending on gel type) between 90 bp- 50kb. For protein, the system uses SDS-agarose to collect targets between 18-200kDa. In both cases, a higher quality of sample is extracted, and more reproducibly, when compared to manual gel purification methods.
SageELF and BluePippin Updates for Iso-Seq
This scientific poster provides workflow updates for Sage Science’s SageELF and BluePippin instruments, optimized for full-length transcript sequencing with Iso-Seq.
Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies
At the Icahn Institute for Genomics and Multiscale Biology at Mount Sinai in New York City, technology development expert Robert Sebra, Ph.D., sees tremendous need for long-read, high-accuracy clinical sequencing for use in microbial surveillance, detection of repeat expansions, and more. To meet that demand, he relies on Single Molecule, Real-Time (SMRT®) Sequencing from Pacific Biosciences with BluePippin™ automated DNA size selection from Sage Science. Together, these tools offer a powerful solution and industry-leading read lengths that allow Sebra and other researchers to resolve repeat elements and structural variants, rapidly close microbial genomes, and measure epigenetic marks.
Say Goodbye to Manual Gels: Automated DNA Size Selection Has Arrived
Automated size selection is a superior alternative to manual gel extraction, allowing scientists to save time and money, improve the efficiency of sequencing runs and analysis, and perform new applications with next-gen sequencers. This white paper describes the use of the Pippin Prep and Blue Pippin size selection platforms for automated DNA size selection.
New Automated Systems for Size-Fractionation of Protein Samples
This application note describes two new systems for automated preparative electrophoresis of protein samples. It is demonstrated that these systems provide increased reproducibility and ease-of-use for procedures that require gel isolation of proteins, such as top-down proteomics studies and other protein mass spectrometry methods. The BluePippin system is useful for applications where a single targeted fraction containing a protein of interest is needed. The ELF system is useful for broader proteomic studies including top-down proteomics and GeLC-MS.
