New Automated Systems for Size-Fractionation of Protein Samples
9 Sept 2013This application note describes two new systems for automated preparative electrophoresis of protein samples. It is demonstrated that these systems provide increased reproducibility and ease-of-use for procedures that require gel isolation of proteins, such as top-down proteomics studies and other protein mass spectrometry methods. The BluePippin system is useful for applications where a single targeted fraction containing a protein of interest is needed. The ELF system is useful for broader proteomic studies including top-down proteomics and GeLC-MS.
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BluePippin Size Selection System
Sage ScienceThe BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples. The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries, or upstream of protein mass-spec. For DNA applications, the BluePippin features pulsed-field electrophoresis to collect high molecular weight targets, and can collect fragments (depending on gel type) between 90 bp- 50kb. For protein, the system uses SDS-agarose to collect targets between 18-200kDa. In both cases, a higher quality of sample is extracted, and more reproducibly, when compared to manual gel purification methods.