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ÄKTA™ go™ protein purification system

Compact liquid chromatography system to support routine protein purification in research laboratories.

Cytiva

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Description

Quickly getting enough protein of desired purity and yield is essential to advance research tasks and get to reliable results. The compact system, with a footprint of only 335 × 464 mm, lets you more efficiently use precious bench and cold cabinet space in your routine column chromatography purifications.

How to develop a protein purification strategy

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Protein purification is rarely a one-size-fits-all process. From recombinant proteins and antibodies to complex protein assemblies, developing an effective purification strategy requires balancing purity, yield, activity, and workflow efficiency.

This SelectScience eBook explores how to develop a structured protein purification strategy using the capture, intermediate purification, and polishing (CiPP) framework. Learn how protein properties, purification objectives, and chromatography selection influence protocol design, and discover practical approaches for combining techniques to maximize separation power while minimizing sample handling.

Through expert guidance, workflow examples, and technique selection tools, you'll gain a clearer understanding of affinity chromatography, ion exchange chromatography, hydrophobic interaction chromatography, and size exclusion chromatography, helping you build more robust and efficient purification workflows.

Key topics explored:

  • How to combine protein purification techniques
  • Optimize your protein purification
  • The CiPP framework in practice
  • Prepacked chromatography columns selection guide
  • Protein research mini-guide

Resource details:

  • Document type: SelectScience guide
  • Page count: 67
  • Read time: 100 mins
  • Edition: 1st


Protein purification troubleshooting guide

To resolve your protein purification challenges, explore detailed troubleshooting tips from Cytiva that cover a variety of issues including pressure and flow rate and retention time, to purity and resolution. Gain practical insights to optimize your protein yields and purity, and accelerate your research.

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