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NEBuilder ® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibilit... Read more...
The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C ) Fast - 5 minutes for cohesive or blunt ends. Convenient - ligation performed at room temperature. ... Read more...
Purifies up to 100 µg of high quality total RNA from multiple sample types - using only one kit Read more...
Taq DNA Polymerase optimized for epigenetics analysis Read more...
Quickly and easily purify high quality DNA from PCR and other enzymatic reactions. Read more...
Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Assembly and transformation in just under two hours Flexible sequence design (scar-less cloning) ... Read more...
The unique workflow of the NEBNext Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol. Read more...
Quickly and easily purify DNA from agarose gels with high yields. Read more...
The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T) 25 to 1 &... Read more...
PvuII has a High Fidelity version PvuII-HF ® (NEB #R3151) High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  ... Read more...
The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression... Read more...
Restriction enzyme digests are now even easier! The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add ... Read more...
Restriction Enzyme Read more...
BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase. Ideal for Plac, Ptac, Ptrc ParaBAD expression... Read more...
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the ... Read more...
The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow. Read more...
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers ... Read more...
Presently, NEB supplies more than 200 restriction enzymes, over 120 of which are available in recombinant form. Read more...
A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging ... Read more...
Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences Read more...
The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Read more...
Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzym... Read more...
For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian) Read more...
NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes an... Read more...
he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). Th... Read more...