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The Cell Motility V2 BioApplication is a advanced image analysis algorithm for measuring the overall motility of cells.  It quantitates total distance traveled by each cell exposed to growth factor or compound for a quantiative assessment of cell motility.  The V2 version includes advanced analytical capabilities such as population analysis and object segmentation.  This application has been biologically validated, enhancing c…

Spot Detector V2 performs measurements of number, size, and other features of spots in cells.  It is an easy-to-use and essential component of any image analysis software suite.  This tool is excellent for measuring receptor internalization and trafficking, cell proliferation and counting, Analyzing populations. This application has been biologically validated, enhancing confidence in your image-based data.  Available for the…

The Cell Spreading V2 BioApplication is an image analysis algorithm for measuring size and shape of cells.  It utilizes cells labeled with cytotskeletal fluorescent reagents to report whether cells have rounded in response to compound treatment, and includes advanced features such as population characterization and object segmentation.  This application has been biologically validated, enhancing confidence in your image-based…

The Neurite Outgrowth V2 BioApplication is a streamlined image analysis algorithm for morphological analysis of neurite outgrowth. Utilizes fluorescent images of neurons and neurites stained for tubulin to quantitate the number and length of neurites in a population of cells.  This application has been biologically validated, enhancing confidence in your image-based data.  Available for the Cellomics ArrayScan VTI, ArrayScan x…

Cyto-Cell Membrane Translocation V2 is a multi-channel image analysis algorithm for analysis of targets that translocate from cytoplasm to cell membrane.  It utilizes colocalization of fluorescent markers to report whether the target is co-localized to the membrane, and reports cells' behavior relative to a control population.   This application has been biologically validated, enhancing confidence in your image-based data.  A…

GPCR Signaling V2 is a high-content image analysis application for quantitation of the activation of G-protein coupled receptors.  Classifies cells into different phases based on pattern of receptor complex distribution and reports details of and percentage of cells with Type A and Type B recycling activity.  This application has been biologically validated, enhancing confidence in your image-based data.  Available for the Cel…

The Multiparameter Apoptosis Kit comprises three spectrally distinct fluorophores that label cellular targets to allow assessment of cell health by looking at several biological parameters.  This kit specifically labels nuclei, f-actin, and mitochondrial mass/potential in cells.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system.  Available in Evaluation Size (Five…

The Multiparameter Cytotoxicity 1 Kit  comprises three spectrally distinct fluorophores that label cellular targets to allow assessment of cell health by looking at several biological parameters.  This kit contains indicators for nuclei, permeabilized cells, and lysosome mass/pH.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system.  Available in Evaluation Size (Five…

Translocation of ATF-2 is measured by an increase in fluorescence in the nucleus relative to the cytoplasm.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for detection with a FITC emission filter.  The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may be us…

The Cell Motility Kit contains fluorescent beads, a cellular marker, and a complete protocol for preparing imaging-quality cells in microplates.  As cells move they make tracks in the beads which can be examined for distance and direction.  The kit has been validated for use with the Cell Motility BioApplicaiton on Cellomics’ ArrayScan® HCS Reader but may be used on any imaging system.  Available in Evaluation Size (Five 96-we…

The Cell Spreading Kit labels the f-actin cytoskeleton and nuclei of cells to enable assessment of cell size, shape, and cytoskeletal structure.  This kit includes fluorescent reagents, buffers, and a complete protocol for preparation of imaging-quality cells in microplates.   The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system.  Evaluation Size (Five 96-well plates).

Phosphorylation of c-jun is measured by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-c-jun with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied…

Activation of ERK can be detected by an increase in phospho-specific fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may b…

The Micronucleus reagent kit comprises three spectrally distinct fluorophores that label cellular targets to allow assessment of genotoxicity/ clastogenicity by looking at several biological parameters.  This kit contains fluorescent probes that label whole cells, nuclei, micronuclei, and permeabilized cells.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system.  Avai…

The Mitotic Index Kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  An antibody to phospho-histone H3 detects cells in early mitosis, and DNA content correlates with intensity of the nuclear-binding dye.  The secondary antibody contains Alexa® Fluor 488.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be app…

The Neurite Outgrowth Kit labels the neuronal form of tubulin to detect neurons and neurites, to enable assessment of neurite outgrowth.  This kit includes fluorescent antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates.   The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five 96-well pl…

Translocation of NF-AT is measured by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applie…

Activation of NFkB can be detected by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applie…

Activation of p38 MAP kinase is measured by an increase in fluorescence in the nucleus of celsl.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-p38 with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be appli…

This reagent kit comprises three spectrally distinct fluorophores that allow assessment of cytoplasm-to-cell membrane translocation of PKC alpha.  This kit specifically labels nuclei (blue), cell membrane (Alexa Fluor(r) 488), and PKC alpha (Alexa Fluor 555) in mammalian cells.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system.  Available in Evaluation Size (Five 9…

Activation of STAT1can be detected by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applie…

Translocation of STAT2 is measured by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applie…

Activation of STAT3 is measured by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may be used on any imagi…

Neuroscreen-1 is a neuronal-like cell line optimized for screening.  It is a subclone of PC12, a widely used cell line derived from rat pheochromocytoma cells that serves as a standard model system for neurons.  Neuroscreen-1 cells display several substantial advantages over PC12 cells, including 50-80% faster growth, no tendency toward cell aggregation, high and accelerated responsiveness to NGF (measurable neurites within 2…

Neuroscreen-1 is a neuronal-like cell line optimized for screening.  It is a subclone of PC12, a widely used cell line derived from rat pheochromocytoma cells that serves as a standard model system for neurons.  Neuroscreen-1 cells display several substantial advantages over PC12 cells, including 50-80% faster growth, no tendency toward cell aggregation, high and accelerated responsiveness to NGF (measurable neurites within 2…