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HitKit® HCS Reagent Kit for p38 MAPK Activation

Activation of p38 MAP kinase is measured by an increase in fluorescence in the nucleus of celsl.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-p38 with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be appli…

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Activation of p38 MAP kinase is measured by an increase in fluorescence in the nucleus of celsl. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-p38 with a FITC emission filter.

The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five 96-well plates) and Screening Size (Fifty 96-well or 200 384-well plates).

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