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HitKit® HCS Reagent Kit for STAT1 Activation

Activation of STAT1can be detected by an increase in fluorescence in the nucleus of cells.  This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates.  The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.  The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applie…

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Activation of STAT1can be detected by an increase in fluorescence in the nucleus of cells. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.

The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five 96-well plates) and Screening Size (Fifty 96-well or 200 384-well plates).

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