HitKit® HCS Reagent Kit for c-Jun Activation
Phosphorylation of c-jun is measured by an increase in fluorescence in the nucleus of cells. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-c-jun with a FITC emission filter. The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied…
Phosphorylation of c-jun is measured by an increase in fluorescence in the nucleus of cells. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-c-jun with a FITC emission filter.
The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be applied to any imaging system. Available in Evaluation Size (Five 96-well plates) and Screening Size (Fifty 96-well or 200 384-well plates).
