HitKit® HCS Reagent Kit for ERK MAPK Activation
Activation of ERK can be detected by an increase in phospho-specific fluorescence in the nucleus of cells. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter. The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may b…
Activation of ERK can be detected by an increase in phospho-specific fluorescence in the nucleus of cells. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal signal with a FITC emission filter.
The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may be used on any imaging system. Available in Evaluation Size (Five 96-well plates) and Screening Size (Fifty 96-well or 200 384-well plates).
