HitKit® HCS Reagent Kit for ATF-2 Activation
Translocation of ATF-2 is measured by an increase in fluorescence in the nucleus relative to the cytoplasm. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for detection with a FITC emission filter. The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may be us…
Translocation of ATF-2 is measured by an increase in fluorescence in the nucleus relative to the cytoplasm. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality, fluorescently labeled cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for detection with a FITC emission filter.
The kit has been validated on Cellomics’ ArrayScan® HCS Reader but may be used on any imaging system. Available in Evaluation Size (Five 96-well plates) and Screening Size (Fifty 96-well or 200 384-well plates).
