Member since: 2014
Organization: Cardiff University
Not the easiest but easy enough and great yield
Application Area: Plasmid purification from E coli cultures
"This is my favourite miniprep kit. It's not the easiest to use, there is a 5-minute incubation step to degrade proteins and therefore more spins than some other kits, but the yield is much better and the kit is a bit cheaper per prep. Nice small box and the reps gave free reagent when I'd spilled a bottle and asked to buy another! "
When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
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