TSKgel mAb SW Gel Filtration Columns for Antibody Analysis
TSKgel mAb SW SEC Columns – Setting new Benchmarks for Antibody Analysis.
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TSKgel mAb SW SEC Columns – Setting new Benchmarks for Antibody Analysis.
TSKgel SW size exclusion columns – the industry’s workhorse for aggregate analysis - continue to set standards for the UHPLC separation of antibody fragments, monomers, and aggregates. TSKgel SW mAb columns are tailored to different aspects of antibody analysis:
- TSKgel SuperSWmAb HTP supports high throughput antibody analysis through a shorter column length and smaller diameter.
- TSKgel SuperSWmAb HR, designed for high resolution monoclonal analysis, is packed in columns featuring the conventional dimensions for HPLC gel filtration columns. This allows for superior resolution between fragments, monomers, and aggregates.
- TSKgel UltraSW Aggregate covers the range of higher molecular weights and provides a very good separation of antibody dimers and higher aggregates. It is based on 3µm silica particles with slightly larger pore size than the SuperSW mAb columns.
TSKgel SuperSW and UltraSW particles are based on highly porous, ultra-pure silica particles, the surface of which has been shielded from interacting with proteins by applying a proprietary surface chemistry. This ensures highest recoveries and accurate quantitation. TSKgel SW columns stand out from other silica-based high performance size exclusion columns by virtue of their large pore volumes. The validated manufacturing and packing process ensures a reliable batch-to-batch reproducibility.
(U)HPLC analysis of biomolecules
This compendium, from Tosoh Bioscience, compiles more than twenty application notes covering important aspects of biopharmaceutical analysis such as protein aggregation, charge isoforms and glycosylation as well as determination of drug-to-antibody ratio of antibody-drug-conjugates. The last section of the notebook provides tips and tricks to improve chromatographic separation of biomolecules.
Size Exclusion Chromatography
Size Exclusion (SEC) is the general name for the chromotographic mode also referred to as gel permeation chromatography (GPC) for non-aqueous elution systems or gel filtration chromatography (GFC) for aqueous systems.
SEC is a method in which components of a mixture are separated according to their molecular size. Large biomolecules that cannot penetrate the pores of the packing material elute first and flow with the mobile phase in the interparticle space of the packed column.
Smaller molecules can partially or completely enter the stationary phase and will elute from the column after the excluded sample components.
SEC is a very simple method for separating biomolecules, because it is not necessary to change the composition of the mobile phase during elution. However, the separation capacity of this method is limited.
