Agilent BioTek Synergy H1 Multimode Reader

There are more than 500 protein kinases, many of which have been shown to be dysregulated in disease states and comprise greater than 30% of all drug developments. In this application note, researchers combine chelation-enhanced fluorescence (CHEF) with the sulfonamido-oxine (Sox) chromophore to measure the activity of protein kinases with a direct, homogeneous and continuous format.

In this application note, new tools designed to optimize cellular metabolism characterization are discussed. One of these tools is a simple mix-and-measure assay compatible with a variety of cellular matrices that utilizes fluorophoric probes to measure oxygen consumption rates (OCR), extracellular acidification (ECA), and intracellular oxygen levels which are useful to inform on the activity of the electron transport chain (ETC) and glycolytic flux. These probes can be detected using standard fluorescence, time-resolved fluorescence, or lifetime fluorescence with reduced background and increased signal dynamic range dependent on the detection mode.

In this application note describes the use of the Lionheart™ FX Automated Live Cell Imager and the ONIX2 Microfluidic Platform to monitor yeast cell growth through the analysis of kinetic brightfield images of yeast cells under a variety of conditions in real time. The yeast under inspection is the yeast Saccharomyces cerevisiae. Strains of the yeast Saccharomyces cerevisiea serve critical roles in the production of many different products including food staples, medicines, and biofuels.

Characterization of cellular metabolism is being aided by the development of new tools designed to provide ease-of-use, higher throughput, and multiplexed data markers for analysis. This application note demonstrates that Luxcel probes can be detected using a variety of BioTek readers to inform cellular metabolic analysis. The results also show that BioTek readers and Luxcel probes are compatible for analyzing extracellular oxygen consumption rates and intracellular O2 levels of live cell response to drug treatment.

The essential amino acid tryptophan is intrinsically fluorescent with excitation in the UV range of the spectrum. This physical characteristic can be used to investigate peptides and proteins that contain this amino acid. This application note describes the use of the Synergy H1 reader in conjunction with UV-capable fluorescence polarizers to investigate conformational and mass changes of proteins in solution.

This application guide discusses the principles of HTRF® detection and proximity assays in general based on time-resolved fluorescence resonance energy transfer. Learn how the Synergy™ product line of Multi-Mode Microplate Readers has been designed specifically for high performance HTRF assays and finally describe some specific applications pertinent to modern drug discovery for both small molecule and biomolecular putative therapeutics.

Here we investigate the performance of a new type of dual luciferase assay using two luciferases with spectrally resolvable light emissions in either 96- or 384-well density microplates using the Thermo Scientific Gaussia-Firefly Luciferase Dual Assay or Gaussia and Renilla Luciferase Flash Assay Kits, respectively. Assay optimization was performed and assay performance was compared across several BioTek Synergy™ Multi- Mode Microplate Readers configured with injectors using lysate containing expressed luciferase enzymes.

There are fluorometric and spectrophotometric methods for nucleic acid quantification. The former is used for applications requiring high sensitivity due to minute available amounts of nucleic acid; the latter is more conventionally used from common nucleic acid extraction procedures. In this application guide discusses spectrophotometric methodology as performed in microplate instrumentation and in specialized micro-volume accessories.

This application note discusses an automated, non-radioactive ADCC assay used in mAb immunotherapeutic development. Results demonstrate that when automated using Biotek instrumentation, the cell-based assay reduces active labor time. In addition, an increase in consistency and robustness are shown over time and across users.

This application note demonstrates a simple, homogeneous, non-radioactive, mix-and-read assay platform for ADCC studies using luminescent detection and genetically engineered cells. The combination of assay chemistry, appropriate cell models, and automated instrumentation can increase productivity and throughput, simplify processes, and generate high-quality, reproducible results for the discovery of new antibody therapeutics.

Biocontrol agents provide a safer alternative to chemical pesticides and insecticides for the agricultural and forestry industries, as well as human health. In this video, Dr Bethany Greenfield, Post-Doctoral Research Assistant in the Biocontrol and Natural Products (BANP) Group at Swansea University, describes her research into the use of Metarhizium anisopliae for the control of mosquitoes. Hear how the BioTek Synergy H1 enables the group to efficiently perform a range of analyses, from protein quantification to measuring insect stress response.

Both multi-mode readers feature a modular architecture so that researchers may purchase based on their immediate needs and upgrade the unit

These monochromators aim to achieve greater levels of assay sensitivity and specificity compared to fixed bandwidth systems