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Seahorse XF Flex Analyzer

The Agilent Seahorse XF Flex analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates in a 24-well format.

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Description

The Agilent Seahorse XF Flex analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates in a 24-well format. It offers optimized workflows and dedicated consumables to enable real-time metabolic analysis with 3D study models, such as organoids and tissue materials. It also provides wide signal dynamic range through the use of dedicated consumables.

OCR, PER, and ATP production rates are critical attributes of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys) function. The ability to analyze these parameters in more complex three-dimensional structures will reveal deeper insights and enhance the translatability of your research.

The XF Flex analyzer is also equipped with advanced software and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.

Features

  • A flexible 24-well system that allows real-time metabolic analysis with improved sensitivity and consistency, using two-dimensional (2D) and three-dimensional (3D) study models
  • Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP production rates
  • Tailored workflows and dedicated consumables for working with organoids and tissue materials
  • Wide detection range and great for high respiration study models
  • Four-port injection system with automated mixing feature for assessing immediate cellular, tissue organoid responses to substrates, inhibitors, and other compounds in real time
  • Verified instrument performance to ensure intraplate and interplate data consistency
  • Broad operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
  • Intuitive, user-guided software for easy assay protocol creation
  • Automated data quality check in data analysis software to flag abnormal assay behavior
  • Data files compatible with advanced data analysis features in the Seahorse Analytics which is a secure web-based data analysis tool

Specifications

Analyzer applicationCellular energy metabolism studies, Phenotyping, 2D cultures, Tissue models, Organoids, Small model organisms
Assay running volume600-1000 µL/well
Assay wells22
Dimensions (WxDxH)38.74 cm x 47.72 cm x 58.42 cm

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