Validation And Comparison Of Single-Step Flash And Dual-Spectral Luciferase Reporter Gene Assays Using The Synergy™ Line Of Microplate Readers

20 Oct 2013

Here we investigate the performance of a new type of dual luciferase assay using two luciferases with spectrally resolvable light emissions in either 96- or 384-well density microplates using the Thermo Scientific Gaussia-Firefly Luciferase Dual Assay or Gaussia and Renilla Luciferase Flash Assay Kits, respectively. Assay optimization was performed and assay performance was compared across several BioTek Synergy™ Multi- Mode Microplate Readers configured with injectors using lysate containing expressed luciferase enzymes.

Agilent BioTek Gen5 Software for Detection

Agilent Technologies

Agilent BioTek Gen5 software for Agilent BioTek multimode and single-mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications. Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.

(83)

Agilent BioTek Synergy H1 Multimode Reader

Agilent Technologies

Agilent BioTek Synergy H1 is a modular multi-mode microplate reader, with monochromator-based optics and filter-based optics. The proprietary Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change.

(21)

Links

Tags