Optimization of a Multi-Mode Detection Model for Measuring Real-Time Cellular Respiration and Mitochondrial Function Using Fluorophoric Biosensors

Characterization of cellular metabolism is being aided by the development of new tools designed to provide ease-of-use, higher throughput, and multiplexed data markers for analysis. This application note demonstrates that Luxcel probes can be detected using a variety of BioTek readers to inform cellular metabolic analysis. The results also show that BioTek readers and Luxcel probes are compatible for analyzing extracellular oxygen consumption rates and intracellular O₂ levels of live cell response to drug treatment.