Spectrophotometric Nucleic Acid Quantification in Microplates and Micro-Volumes

20 Aug 2013

There are fluorometric and spectrophotometric methods for nucleic acid quantification. The former is used for applications requiring high sensitivity due to minute available amounts of nucleic acid; the latter is more conventionally used from common nucleic acid extraction procedures. In this application guide discusses spectrophotometric methodology as performed in microplate instrumentation and in specialized micro-volume accessories.

Agilent BioTek Epoch Microplate Spectrophotometer

Agilent Technologies

The Agilent BioTek Epoch microplate spectrophotometer has monochromator-based optics, for a filter-free, wide wavelength range for UV-Vis absorbance measurements. These measurements can be done in many microplate formats and in 2 µL samples when the available Take3 microvolume plate is used.

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Agilent BioTek Synergy H1 Multimode Reader

Agilent Technologies

Agilent BioTek Synergy H1 is a modular multi-mode microplate reader, with monochromator-based optics and filter-based optics. The proprietary Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change.

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Spectrophotometric Nucleic Acid Quantification in Microplates and Micro-Volumes