The Seismos NT.X offers a surface-based method to measure binding-induced conformational changes and binding kinetics. Since Surface Acoustic Wave (SAW) is working acoustically, it overcomes the limitations of optical biosensor technologies, such as colored and turbid samples.
SAW technology quantifies changes in mass and conformation separately and offers new insights into molecular interaction mechanisms. Binding kinetics (kon, koff, and Kd) can be investigated for standard interactions as well as for complex samples including viruses, vesicles and membrane preparations.
The SAW technology is based on the precise detection of the properties of surface acoustic waves that travel along the biosensor. Upon interaction with molecules on the sensor surface, distinct characteristics of the acoustic waves are altered:
Both signal types are detected separately and can be used to comprehensively characterize the interaction mechanism of the molecules on a kinetic and structural level.