RoboGene HIV-1 RNA Quantification Kit (RUO) by Analytik Jena AG

Manufacturer Analytik Jena AG
The RoboGene® HIV-1 RNA Quantification Kit is intended for real-time quantification of Human Immunodeficiency Virus type 1 (HIV-1) RNA in human EDTA plasma and serum. The level of HIV-1 RNA in serum and plasma can be used in conjunction with other clinical markers and clinical findings to distinguish between acute and chronic HIV-1 infection and to assess the viral response to antiviral treatment. Procedure During sample preparation a synthetic internal control is included via Extraction tub... Read more


RoboGene HIV-1 RNA Quantification Kit (RUO) by Analytik Jena AG product image
RoboGene® HIV-1 RNA Quantification Kit (RUO)
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The RoboGene® HIV-1 RNA Quantification Kit is intended for real-time quantification of Human Immunodeficiency Virus type 1 (HIV-1) RNA in human EDTA plasma and serum. The level of HIV-1 RNA in serum and plasma can be used in conjunction with other clinical markers and clinical findings to distinguish between acute and chronic HIV-1 infection and to assess the viral response to antiviral treatment.

Procedure
During sample preparation a synthetic internal control is included via Extraction tubes RNA_D1 to control RNA extraction and to indicate for inhibitory effect on detection. Quantitation standard consists of 8 tubes coated with given amounts of synthetic HIV RNA, which must be amplified in parallel. Amplification of HIV RNA in samples and standards and of IC RNA is measured independently at different wavelengths due to probes labelling with different fluorescence reporter dyes.