ReliaPrep™ RNA Miniprep Systems
The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or fro…
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Good quality isolation from valuable or precious samples (stem cells)
Neurosciences
Kit for efficient isolation of RNA from cultured stem cells, good for valuable samples to obtain high yield of quality material
Review Date: 27 Oct 2025 | Promega Corp.
Easy and quick and good price!
RNA extraction
I have used this kit for extracting RNA from a variety of 2D and 3D cell culture. It is easy to use and quick! The price beats competitor's kits. Definitely reccomended.
Review Date: 22 Jul 2021 | Promega Corp.
The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes.
The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or from 0.25 to 20mg of tissue can be processed per purification.
ReliaPrep™ RNA Miniprep Systems Features & Benefits:
- Be Efficient - Allows use of hard-to-obtain precious samples.
- Have Confidence - Provides maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
- Save Effort - No need to further concentrate samples for use.
- Save Time - Rapid protocol and provided DNase reagents streamline laboratory processes.
RNA Isolation from 3D Microtissues Using the Promega ReliaPrep™ RNA Tissue Miniprep System
This application note details a protocol to isolate total RNA from as little as 1 microtissue (~ 1000 cells) using the ReliaPrep™ RNA Tissue Miniprep System. Isolation of high quality total RNA from cultured cells is important for various analyses in cell-based drug discovery and toxicity. Analytical applications such as quantitative real time PCR or microarray analysis depend on robust RNA isolation procedures.
Validation of In Vitro Assays to Measure Cytotoxicity in 3D Cell Cultures
This poster presents the results of experiments designed to improve performance of assays for 3D spheroids. The lytic capacity of assay reagents was enhanced by optimizing the detergent concentration and the parameters for physical disruption necessary to extract the desired markers. Improved protocols and reagents are described to measure ATP as a cell viability marker, caspase-3/7 activity as an apoptosis marker, glutathione as a marker of oxidative stress and HIF-1 promoter driven expression of luciferase as a hypoxia marker.
