QuantiTect Rev. Transcription Kit (200) by QIAGEN
Place your order directly with the manufacturer.
Status:
Reviewer
Member since: 2018
Organization: University of Bristol
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- Ease of use
- After sales service
- Value for money
Easy to use and good results.
Application Area: Reverse transcribe RNA to cDNA for qPCR
"Easy to use kit, very low variation between quantity of sample reversed, some of the reagents are not given in adequate amounts which can be frustrating as the RT (red tube) always seems to run out first while the rest of the reagents are still plentiful."
Review date: 14 Dec 2018 | QuantiTect Rev. Transcription Kit (200)
Status:
Advanced Reviewer Member since: 2015
Organization: Columbia University
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- Ease of use
- After sales service
- Value for money
Great results easy to use
Application Area:Prepare cDNA
"Highly reproducible results Easy to use kit Works well with all volumes of RNA"
Review date: 29 Jun 2016 | QuantiTect Rev. Transcription Kit (200)
Status:
Reviewer Member since: 2013
Organization: Columbia University
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- Ease of use
- After sales service
- Value for money
Great kit, well worth the price.
Application Area:reverse transcription of RNA to produce cDNA
"Good value for money. High quality product with reliable and reproducible results. Little to no variation from lot to lot. Consistent quality of cDNA is obtained. The genomic wipe-out buffer eliminates most genomic DNA and so DNAse treatment is not even required."
Review date: 04 Dec 2015 | QuantiTect Rev. Transcription Kit (200)
QuantiTect Reverse Transcription Kit. For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis.
Kit Contents:
Qiagen QuantiTect Reverse Transcription Kit, 200 x 20L rxns, RNA Template Sample, Two-step, cDNA Production, Genomic DNA Digestion Reaction Type, For Fast cDNA Synthesis Enabling Sensitive Real-time Two-step RT-PCR for Gene Expression Analysis, Includes 4 x 100L 7x gDNA Wipeout Buffer, 4 x 50L Quantiscript Reverse Transcriptase, 4 x 200L 5x Quantiscript RT Buffer, 4 x 50L RT Primer Mix, 4 x 1.9mL RNase-free Water.
Benefits:
- cDNA synthesis and gDNA removal in only 20 minutes
- High cDNA yields even from low-abundance transcripts
- cDNA synthesis from 5' and 3' regions of transcripts
- No need to design RNA-specific primers or probes
