Prometheus Panta for AAV stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies by NanoTemper Technologies

Manufacturer NanoTemper Technologies  |  Available Worldwide
Prometheus Panta measures essential stability parameters of your AAVs to support serotyping, formulation, and production of your viral vectors for gene therapy — providing  you with information about stability, purity, and capsid assembly in one experiment.  Prometheus Panta combines DLS, SLS, nanoDSF, and backreflection technologies to give you detailed insight into  your AAV vector’s stability profile.  


Prometheus Panta for AAV stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies by NanoTemper Technologies product image
Prometheus Panta for AAV stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies
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Features:

  • Measure thermal unfolding, particle sizing, molecular weight, and aggregation simultaneously, throughout an entire thermal ramp
  • High-resolution data about your AAV’s stability profile
  • Flexible throughput, with multiple options for sample handling

Parameters that Prometheus Panta measures:

Dynamic Light Scattering (DLS)

 

SIZE ANALYSIS

 

Tsize (from growth of rH in cumulants fit): Temperature at which average particle size begins to increase, indicating unfolding

Average scattering intensity: Identifies if concentration is too high or low for proper size distribution analysis

 

CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS

rH: Hydrodynamic radius, shows size of particle in solvated state

PDI: Polydispersity index represents distribution of size populations, a low value is indicative of properly assembled capsids without contaminants

SELF-INTERACTION ANALYSES

kD: Self-association constant, a parameter used to determine the propensity of a particle to aggregate at higher concentrations

D0 (*derived from kD analysis): Theoretical diffusion constant at concentration = 0

 

nanoDSF

 

THERMAL UNFOLDING

 

Tm (for 330 nm, 350 nm, and ratio): Melting temperature, the point at which 50% of the viral particle proteins that comprise your capsid are unfolded; Tm values are a hallmark of certain serotypes, you can also see how small changes to capsid proteins affect their overall stability, as higher Tm values are considered more stable

Tonset/Ton (for ratio): Temperature at which unfolding begins; higher Ton values indicate increased thermal stability, and it is considered favorable to have a sharper slope at the unfolding inflection point, which occurs when the Ton is close in value to the Tm

Ea (*parameter derived from thermal unfolding data): Activation energy of unfolding, a parameter that informs on the stability of a protein in a given buffer environment

Reversibility of unfolding: Point at which aggregated capsid proteins cannot be recovered and refolded without other chemical means

 

Backreflection

 

AGGREGATION

 

Tturbidity: Onset temperature of large (>12.5 nm radius) aggregate formation


SLS

SIZING INFORMATION

 

Molecular weight: Average molecular weight of all particles in solution; requires a homogenous preparation of a given sample to maximize accuracy

SELF-ASSOCIATION AND AGGREGATION PROPENSITY

Tscattering: Temperature at which scattering intensity begins to change, indicating the increased size and smaller aggregate formation

B22: Second virial coefficient, used to extrapolate self-association behavior at higher concentrations

Product Overview

Prometheus Panta for AAV stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies by NanoTemper Technologies product image

Prometheus Panta for AAV stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies

Manufacturer NanoTemper Technologies  |  Available Worldwide

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