Features:
Parameters that Prometheus Panta measures:
Dynamic Light Scattering (DLS)
SIZE ANALYSIS
Tsize (from growth of rH in cumulants fit): Temperature at which average particle size begins to increase, indicating unfolding
Average scattering intensity: Identifies if concentration is too high or low for proper size distribution analysis
CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS
rH: Hydrodynamic radius, shows size of particle in solvated state
PDI: Polydispersity index represents distribution of size populations, a low value is indicative of properly assembled capsids without contaminants
SELF-INTERACTION ANALYSES
kD: Self-association constant, a parameter used to determine the propensity of a particle to aggregate at higher concentrations
D0 (*derived from kD analysis): Theoretical diffusion constant at concentration = 0
nanoDSF
THERMAL UNFOLDING
Tm (for 330 nm, 350 nm, and ratio): Melting temperature, the point at which 50% of the viral particle proteins that comprise your capsid are unfolded; Tm values are a hallmark of certain serotypes, you can also see how small changes to capsid proteins affect their overall stability, as higher Tm values are considered more stable
Tonset/Ton (for ratio): Temperature at which unfolding begins; higher Ton values indicate increased thermal stability, and it is considered favorable to have a sharper slope at the unfolding inflection point, which occurs when the Ton is close in value to the Tm
Ea (*parameter derived from thermal unfolding data): Activation energy of unfolding, a parameter that informs on the stability of a protein in a given buffer environment
Reversibility of unfolding: Point at which aggregated capsid proteins cannot be recovered and refolded without other chemical means
Backreflection
AGGREGATION
Tturbidity: Onset temperature of large (>12.5 nm radius) aggregate formation
SLS
SIZING INFORMATION
Molecular weight: Average molecular weight of all particles in solution; requires a homogenous preparation of a given sample to maximize accuracy
SELF-ASSOCIATION AND AGGREGATION PROPENSITY
Tscattering: Temperature at which scattering intensity begins to change, indicating the increased size and smaller aggregate formation
B22: Second virial coefficient, used to extrapolate self-association behavior at higher concentrations
Manufacturer NanoTemper Technologies | Available Worldwide
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