Member since: 2019
Organization: University of Colorado, School of Medicine, USA
My go-to column for purification of proteins
Application Area: biomolecules purification
"I'm always having his-tagged protein to purify and this is my go-to column. As the column seems to be losing the blue/Ni color quite fast, it's always important to strip and recharge. The column comes with clear instructions on using and recharging."
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
High binding capacity, approx. 40 mg/mL resin.
Negligible leakage of Ni2+.
Prepacked columns offer reliable and convenient time-saving purification of histidine-tagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.