The Monolith NT.LabelFree measures biomolecular interactions via MicroScale Thermophoresis (MST).
Thermophoresis is detected and quantified using intrinsic tryptophan fluorescence and thus provides a truly label-free experimental setup. Without the need for a fluorescent label or any surface immobilization, the interaction studies performed with the Monolith NT.LabelFree reflects the binding of the protein in its native state. Combined with low sample requirements, label-free MST allows for the measurement of susceptible proteins which are difficult to handle and which are typically available in limited quantities.
Besides the dissociation constant, MST datasets contain unique information about aggregation and sample quality allowing for rapid assay setup and straightforward sample optimization.
Interactions between any kind of biomolecules, from single ions, fragments, and small molecules up to high molecular weight complexes.