LigaFast™ Rapid DNA Ligation System by Promega Corp.

Manufacturer Promega Corp.
4.3
/
5.0
  |  1 reviews
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligati... Read more


LigaFast™  Rapid DNA Ligation System by Promega Corp. product image
LigaFast™ Rapid DNA Ligation System
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: The University Of Nottingham



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
Time saver!!
Rating: 4.3

  • Application Area: Molecular cloning into synthetic bacteria

"When time isn't on my side in the lab, this ligase is a life saver. After an easy reaction setup and only a few minutes I have a high proportion of ligated fragments, for which I can transform into my E.coli. Meaning in a day I can have my recombinant plasmids constructed and in my bacteria, opposed to 2 days with other standard ligases."

Review date: 02 Jul 2019 | LigaFast™ Rapid DNA Ligation System
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).

Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.


LigaFast™ Rapid DNA Ligation System Features & Benefits:

  • Flexible - Use with 5´, 3´ or blunt-ended DNA inserts.
  • Fast - Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.
  • Convenient - No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature.
  • Ready-To-Use - No additional buffer modifications required prior to use.
  • Efficient - Ligations performed using the LigaFast™ System are comparable to standard overnight ligations.
  • Blue/White Cloning Qualified - Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.

Product Overview

LigaFast™  Rapid DNA Ligation System by Promega Corp. product image

LigaFast™ Rapid DNA Ligation System

Manufacturer Promega Corp.

4.3 / 5.0 | 1 reviews