Human HGF ELISA by RayBiotech Inc.

Manufacturer RayBiotech Inc.
4.3
/
5.0
  |  1 reviews
Product Features Sensitivity: 3 pg/ml Detection Range: 3 pg/ml - 2000 pg/ml Recommended Dilution (serum & plasma): 2 fold Additional Notes Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha,... Read more


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Human HGF ELISA
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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Whitehead Institute for Biomedical Research



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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HGF elisa kit
Rating: 4.3

  • Application Area: serum tests from animals

"The kit is easy to use. worked as expected with positive and negative controls. The instructions are clear and follow step by step applications even for first timers in ELISA. comes in stripes so you don't have to use all 96 wells at a time"

Review date: 20 Jul 2017 | Human HGF ELISA

Product Features

Sensitivity: 3 pg/ml

Detection Range: 3 pg/ml - 2000 pg/ml

Recommended Dilution (serum & plasma): 2 fold

Additional Notes

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Species Detected: Human

Protein Name & Synonyms: Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF)

Accession Number: P14210

Gene Names: HGF, HPTA

Gene ID: 3082

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Other Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

  • Prepare all reagents, samples and standards as instructed in the manual.
  • Add 100 µl of standard or sample to each well.
  • Incubate 2.5 h at RT or O/N at 4°C.
  • Add 100 µl of prepared biotin antibody to each well.
  • Incubate 1 h at RT.
  • Add 100 µl of prepared Streptavidin solution to each well.
  • Incubate 45 min at RT.
  • Add 100 µl of TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl of Stop Solution to each well.
  • Read at 450 nm immediately.

Storage/Stability: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.