ExoSAP-IT PCR Product Cleanup Reagent by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 78205.10.ML  |  Available Worldwide
4.3
/
5.0
  |  1 reviews
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.


ExoSAP-IT PCR Product Cleanup Reagent by Thermo Fisher Scientific product image
ExoSAP-IT PCR Product Cleanup Reagent
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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


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  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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The best way to prepare PCR products for Sanger sequencing!
Rating: 4.3

  • Application Area: Sample preparation for sanger sequencing

"ExoSAP-IT is an enzyme mixture used to "clean up" PCR products (i.e., digest PCR primers) so they can be directly sequenced by the Sanger dideoxynucleotide method following addition of sequencing primer. It provides a simpler alternative to silica column clean up, and requires less hands-on time (although a half hour 37C-80C incubation in a thermal cycler is required). Note that the volumes of ExoSAP-IT and PCR product specified by the manufacturer can be scaled down 5-fold or so, making it very cost-effective (<$1 per sample). Most importantly, the sequence chromatograms are consistently excellent (depending on your PCR product, of course)."

Review date: 22 Jan 2021 | ExoSAP-IT PCR Product Cleanup Reagent

ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.

  • Remove excess primers and unincorporated nucleotides enzymatically
  • Prepare DNA sequencing samples with one pipetting step
  • Conserve precious PCR products with up to 100% recovery
  • Minimize column cleanup and sample loss


The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.

Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.

After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.

Product Overview

ExoSAP-IT PCR Product Cleanup Reagent by Thermo Fisher Scientific product image

ExoSAP-IT PCR Product Cleanup Reagent

Manufacturer Thermo Fisher Scientific  |  Available Worldwide

4.3 / 5.0 | 1 reviews