Status:
ReviewerMember since: 2023
Organization: Uni of Manchester
Cheap and quick synthetic DNA
Application Area: Synthetic DNA fragment in plasmid
"Review for 2.5kb synthetic DNA fragment in plasmid: Chosen for low price and quick turn around. Reps were helpful. The DNA sequence ordered included a section of DNA that some competitors wouldn't synthesise."
Status:
ReviewerMember since: 2023
Organization: TriptoBIO
Very satisfied with the services provided.
Application Area:Test of several genes involved in the biosynthetic pathway
"The whole process (from costumer support/implementation to actual acquisition of batches of genes) was very smooth and effective. The costumer support personnel was always available to answer my questions and solve all issues. The products have fair prices and the company met the expectations both in quality of the product and estimated delivery times."
Status:
ReviewerMember since: 2021
Organization: Orion Corporation
Twist makes using synthetic DNA constructs easy and affordable
Application Area:Overexpression of recombinant proteins
"Ordering synthetic DNA is very easy through the Twist eCommerce, the products arrive quickly and work as they should."
Status:
ReviewerMember since: 2023
Organization: UCLA-DOE Institute
My favorite for gene synthesis
Application Area:Synthetic DNA
"I've been using Twist Bio for gene synthesis since 2018. Pricing is excellent and the the turnaround time is very reasonable (you can get gene fragments from other suppliers faster but at a higher cost. ) I usually have them clone into a vector, for a very reasonable cost, so I can go directly to expression studies. They have a decent selection of bacterial and mammalian expression vectors as well as cloning vectors. You also have the option to send them a vector if you need something other than what they offer (e.g. insect cell expression)."
Status:
ReviewerMember since: 2023
Organization: University of birmingham
Great gene synthesis service
Application Area:Structural biology
"The gene synthesis is relatively easy, and the cost is very good. The turnaround time is very short too."
Status:
ReviewerMember since: 2023
Organization: LUMC
Recommended; great service for price.
Application Area:Protein expression
"Design is a breeze, in particular when you supply an amino acid sequence for codon optimization. Generally reasonable expression for all constructs ordered to date. Quick turnaround, and easy to follow progress of the order."
Status:
ReviewerMember since: 2023
Organization: University of Toronto
Great value, great customer service
Application Area:Building yeast strains using genes synthesized and subcloned into custom vector by Twist
"I am a graduate student working on a project which requires the construction of hundreds of yeast strains, each carrying a plasmid with a different gene inserted in the plasmid. Twist has saved me an enormous amount of work by offering to not only synthesize these genes, but also subcloning them into the custom vector I would like to use, all for a very reasonable price! Based on my experience with their customer service team, it is evident that Twist truly cares about their customers and the quality of their products/services. Would strongly recommend choosing Twist for all your gene synthesis and cloning needs."
Status:
ReviewerMember since: 2023
Organization: Freie Universität Berlin
Very good and fast service.
Application Area:Molecular cloning for protein expression
"I heard Twist Bioscience from one of my friends. I was trying to clone two new protein constructs for my project, they are quite time-consuming. Then we decided to synthesize them. My friend told me that he usually orders genes from Twist Bioscience. I compared their prices with other companies and found them to be quite reasonable. My friend told me that they are quite reliable, so we decided to order genes from Twist Bioscience. We placed the order before the Christmas holiday and received the products after New Year. It was pretty fast. We are quite satisfied with their service."
Status:
ReviewerMember since: 2023
Organization: Moredun Research Institute
Rapid service and excellent pricing.
Application Area:Synthetic gene synthesis
"I recently purchased 15 synthetic genes from Twist Bioscience. All genes were designed and codon optimised using the Twist website which was easy and intuitive to use. Genes were ordered just before the Christmas break so I wasn't expecting them to be deliered to the UK from the US until sometime in January, however all genes were delivered a couple of days after Christmas which was an impressive turnaround (I've previously waited up to 3 months with competitors). The quality and yield of all genes was excellent and I've transformed 5 of the synthetic gene clones into E. coli so far with ease. Pricing from Twist was exceptional, I paid less for these 15 genes than I have previously paid for 6 genes from a competitor. Highly recommend Twist"
Status:
ReviewerMember since: 2022
Organization: St. Jude Children's Research Hospital
Very Good
Application Area:Synthetic genes
"My sales rep (Catherine Sutton) is an extremely friendly and helpful person who will do everything to offer you a good quality product at a reasonable price."
Status:
ReviewerMember since: 2015
Organization: University of Cologne
My new way of cloning
Application Area:protein expression in bacteria
"Twist is very easy to use, has a very helpful design tool for plasmid design, the costs are very reasonable and the turnaround time is very good. Highly recommend."
Status:
ReviewerMember since: 2022
Organization: Arizona State University
Fast, Reliable Plasmids at a not so steep price tag
Application Area:Ordering synthetic genes inserted into plasmids
"Here's the deal, Twist is, to my knowledge just, simply getting this work done faster than anyone in the field. I frequently (although not always) only wait two weeks from the time I order until the plasmids hit the lab. This timeframe is extremely helpful and allows us to iterate in our workflow significantly faster. I would not say Twists services are necessarily cheap, but they provide high-quality work you can trust. Which in the end may save you even more time and possibly headaches. They could use some more variation for their in-house e. coli plasmids, but the ones they have are sufficient."
Status:
ReviewerMember since: 2021
Organization: Barts and The London School of Medicine and Dentistry, Queen Mary University of London
Twist has changed the way we work, we are more productive
Application Area:Antibody Engineering
"Twist has made our research very productive. We can design molecules and Twist rapidly produce the recombinant constructs to test our ideas. Todate we have done many projects with Twist and are happy with each and everyone. Wonderful on-line support and follow up."
Status:
ReviewerMember since: 2022
Organization: POPVAX PVT LTD
Lightening speed gene synthesis under 5kb
Application Area:Molecular biology
"Twistbio is one of the best platform for gene synthesis considering time and budget. Only glitch is that it doesn’t accept highly repeated sequences. The team is readily available if any help required with the service."
Status:
ReviewerMember since: 2022
Organization: New England Biolabs
Easy and affordable
Application Area:Biotechnology
"Twist's clonal products were easy to use. The web portal was simple to find vectors for my desired insert and easy to check for frameshifts. The cost can't be beat either!"
Status:
ReviewerMember since: 2022
Organization: Amgen BC
Cloning by mail
Application Area:Protein Sciences
"I have been using Twist cloned gene service for a couple of projects. It was easy to order on Twist's website. The cost and TAT are great. Cloning yourself and sending it for sequencing is more expensive than ordering the construct from Twist. They also NGS verify the clone. Customer service is excellent - You get a knowledgeable service team member within minutes, and they are always happy to help troubleshoot."
Status:
ReviewerMember since: 2022
Organization: UCSF
We got our plasmids way sooner than anticipated
Application Area:Expression plasmid for cell transfection
"We asked Twist Bioscience to synthesize two expression plasmids carrying a 300 bp cDNA each, coding for small proteins. The original delivery date was 17 working days post-order placement, but as the insert was really short and as we needed data quickly for inclusion in a funding proposal, we asked if it would be possible to get them faster. Not only it was easy to communicate via the chat feature of the website, but people were consistently useful in updating us on progress. Also, our request was taken seriously (no lip service) and, low and behold, we got our plasmids way sooner than anticipated! Great service all around!"
Status:
ReviewerMember since: 2022
Organization: UTEC University
Good price & time of delivery
Application Area:Gene synthesis
"I recently switch to Twist, I used to buy genes from Genscript; but the price it is really good and the time of delivery is reasonable being myself in Peru. Thanks!"
Status:
ReviewerMember since: 2022
Organization: University of Michigan
Fast production and delivery times - Friendly customer support
Application Area:I'm using the clones I ordered to express and purify a protein that was hard to obtain
"They have a variety of plasmids to choose from which saves steps for downstream processing. The software for optimizing the sequence is very intuitive and has a lot of checks built in that help you catch errors in your sequence. I almost forgot to add ATG to my protein! Luckily the software caught it for me. I was able to optimize codons for my model organism which is a great feature."
Status:
ReviewerMember since: 2022
Organization: University of Wisconsin-Milwaukee
Great way to save time and money!
Application Area:Developmental genetics
"The clonal gene service from Twist Bioscience is a great time saver. In addition, it makes it possible to optimize DNA sequence to maximize protein expression in a way that was not previously practical/cost effective. One of the most useful aspects of the service is that you can have your gene cloned into your own custom vector. The service is very cost effective because it covers synthesis, cloning and sequence verification all at once. One area for improvement would be if they would set up an option to allow the synthesized DNA from a single order to be cloned into multiple vectors. This would make the service even more cost effective. One other area for improvement would be if the technology could be more tolerant of sequence complexities, which sometimes make it impossible to synthesize a needed DNA sequence (this is especially true of promoters and UTRs, which can not be codon optimized)"
Status:
ReviewerMember since: 2022
Organization: Tel Aviv University
Great results that save time
Application Area:Expression genes in plant for genome editing
"We use the Twist clonal gene as part of a biosynthesis gene pathway to edit the genome in plants. The ability to change the codon usage from E Coli to Plant codon usage was very helpful."
Status:
ReviewerMember since: 2022
Organization: University of South Carolina College of Pharmacy
Generated constructs work as expected. We received very promising preliminary results
Application Area:Gene synthesis for functional analysis of the generated polymorphic variants
"We received a very important tool for our research project. It was quick and cost-efficient."
Status:
ReviewerMember since: 2022
Organization: BC Cancer
Convenient, consistent service for high throughput cloning.
Application Area:Custom human recombinant antibody production
"It was easy to onboard our vectors and now it is very easy to clone any sequence we want into them. We always get clones from the glycerol stocks and DNA yields are typically quite high. In this most recent run, now sequences failed production and required re-preps (which are always included regardless) so all 55 vectors were delivered before the ETA which was fantastic!"
Status:
ReviewerMember since: 2022
Organization: ZIS Sweetening Ltd.
Great service
Application Area:Foodtech
"We enjoyed the service, the product arrived quickly and we managed to use it without any problems later."
Status:
ReviewerMember since: 2022
Organization: INRAE
Easy to design gene constructs, easy to order, cheap, good time of production
Application Area:Protein-protein interactions studies
"As a starting point of our European project, we have used Twist Bioscience gene synthesis service to clone > 80 ORFs encoded by African swine fever virus into the pTwist ENTR vector. From this collection, we next took advantage a versatile recombination-based cloning strategy to shuffle the viral ORFs into different vectors and express recombinant proteins using various systems."
Status:
ReviewerMember since: 2022
Organization: Armata Pharmaceuticals
Frustrating design tool but an immensely useful service!
Application Area:Synthetic biology
"Twist delivered the ordered cloned DNA fragments in good times and all were faithful. The ordering process was a bit frustrating because the design principles for acceptable constructs are not super clear but I eventually managed to find a compromise that passed scoring. I like the wide array of available backbones though I would hope for a broad host range plasmid (pBBR1 or RK2). I guess, I'll have to remedy that by sending them my favorite plasmid next time. I will order again as it saves a lot of time to outsource cloning."
Status:
ReviewerMember since: 2022
Organization: Scripps Institution of Oceanography
Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable
Application Area:In vitro enzymatic assays
"Clonal genes from Twist are essential to our lab work involving heterologous expression and in vitro enzymatic assays! Twist makes it easy to order, codon optimize the genes, and the prices are unbeatable. The representatives from Twist were also responsive and helpful throughout the ordering process."
Status:
ReviewerMember since: 2022
Organization: Instituto de Productos Lacteos de Asturias (IPLA-CSIC)
Great results at low prices
Application Area:Expression of proteins
"The gene cloning service was easy to use, accurate and production time was very fast, also delivery even if it would arrive to europe. Quality of DNA was high and it was easy to recover after transformation. The value service is very high it has very competitive prices compared with ther options."
Status:
ReviewerMember since: 2022
Organization: NabGen Technology
Excellent in every way
Application Area:Quick diagnosis
"We systematically order our genes from Twist because of: The very simple use of the website, the reactivity, professionalism and kindness of the agents, the speed of gene synthesis and delivery, and the most important, the clonal genes or gene fragments ordered have always been of excellent quality."
Status:
ReviewerMember since: 2022
Organization: Carl R. Woese Institute for Genomic Biology, UIUC
Support team give valuable comments and suggestion.
Application Area:Protein engineering
"Very efficient and user friendly. Support team give valuable comments and suggestions."
Status:
ReviewerMember since: 2022
Organization: Nottingham Trent University
Great web interface, quick expert communication and fast synthesis
Application Area:Protein expression
"Gene synthesis at this scale and speed allowed my group to screen quickly several protein targets and identify the front runners for further functional studies. I will use the service routinely and I have recommended it already to colleagues."
Status:
ReviewerMember since: 2022
Organization: Massachusetts Institute of Technology
Excellent and cheap gene production service
Application Area:Custom-made gene for enzyme production
"Order genes from them often, first time ordering full clonal service. Products are always excellent (perfect sequences), best value for money (much cheaper than most other services), and decent turnaround time (this time even 3 days earlier than promised!). Gene design (codon optimization, restriction sites etc) is very easy on their platform, full construct with vector somewhat more cumbersome. Plenty of useful add-ons (removal of adapter sequences, glycerol stock preps) at reasonable prices. Can see their service constantly improving. Chat customer support is quick and helpful."
Status:
ReviewerMember since: 2022
Organization: University of Bari, Department of Biosciences, Biotechnologies and Biopharmaceutics
The best gene synthesis service I have ever used!
Application Area:Cloning of a codon-optimized gene
"Twist Bioscience provides a very easy and flexible system to optimize and order your synthetic gene. The customer service is always extremely fast to answer your questions. Price is very competitive. I strongly recommend this service."
Status:
ReviewerMember since: 2021
Organization: University of Birmingham
Excellent service. Reliable and cost effective.
Application Area:Gene synthesis and cloning.
"Twist have great customer service. Any time I’ve had an issue they have solved it very quickly. They are also fast and competitively priced."
Status:
ReviewerMember since: 2021
Organization: Bar-Ilan University
Great service, will absolutely order agian!
Application Area:Transfection for exogenous expression of genes of interest
"We ordered cloned genes for exogenous expression. For proper synthesis and cloning, Twist has an easy-to-use interface that scores your sequence and allows specific changes to be made. They also have a codon optimization tool that allows you to pick specific regions that you don't wish to change, which was really important in our case. Twist also has very reliable customer service that is available for any question. The quality of the plasmids is very good, we received high amounts of them and it's easy to use."
Status:
ReviewerMember since: 2021
Organization: UT Austin
Will order again
Application Area:Protein engineering
"Using the website was unexpectedly easy, my sequences arrived on time and without errors."
Status:
ReviewerMember since: 2021
Organization: Okinawa Institute of Science and Technology
Synthetic sequences of very high quality, excellent customer service.
Application Area:Recombinant expression of human genes
"I ordered multiple recombinant human genes codon optimized and with tags. The genes were successfully synthesized and after cloning, I verified through sequencing that there was not a single mutation in any of the genes. Furthermore, in spite of initial trouble during cloning, Twist offered to retry cloning at no additional charge, including a specially long gene."
Status:
ReviewerMember since: 2021
Organization: Stanford
Fast, sequence verified, synthetic genes in a plasmid, ready for immediate testing.
Application Area:Gene synthesis and cloning
"Really enabling as a bench scientist to simply order sequence verified plasmids containing our synthetic biology genetic designs and get a tube with plasmid ready for immediate delivery to mammalian cells. Good turnaround - helped me figure out quickly if a new project would be feasible."
Status:
ReviewerMember since: 2021
Organization: ARO, Volcani center
Wonderful results.
Application Area:Cloning into a plant expression vector
"I got three tubes with DNA. resuspended it, cut it and cloned it into my vector."
Status:
ReviewerMember since: 2021
Organization: University of Helsinki
Overall, great service, thumbs up from me!
Application Area:Organelle biogenesis and development
"I have used Twist Bioscience to purchase gene fragments in the past and recently for clonal gene purchase. The service was quick and easy, although the site seemed a bit complicated at first. However, thanks to forthcoming staff from the online chat I managed my purchase without any issues. Upon arrival products were easy to use, as they contained enclosed instruction on how to proceed (e.g. if product is delivered dry and requires dissolving). In terms of yield, both times the amount was closer to higher than lower amount as suggested upon purchasing, which is also good. Sequencing for both types of products resulted in exact match."
Status:
ReviewerMember since: 2021
Organization: FALCONBIO
The price is great and the web-interface is intuitive.
Application Area:Synthetic biology
"I enjoyed the web interface for cloning genes and the price of synthetic DNA."
Status:
Advanced ReviewerMember since: 2021
Organization: Philipps University Marburg
Great product, cost effective.
Application Area:Recombinant protein production
"I ordered the clonal gene for my recombinant protein expression in E.coli. The subcloning was fast in my desired vector and half the price of the other company. I am happy with the service and products and have been a regular customer since 2020. I would definitely order all my clonal genes from Twist bioscience."
Status:
ReviewerMember since: 2021
Organization: Binomica Labs
Awesome parts, very affordable even for amateur biologists budget!
Application Area:Plant genetic engineering and amateur biology
"I've been buying Twist genes both clonal and fragments since late 2017. The customer service has been great and products have always been exactly as requested. Shipping times are quick even during a pandemic where most just did not ship, they kept a 17 day turnaround which all things considered is lightning fast. Seamless ordering process and easy to use website with no excess cruft that other synth houses have. Highly recommending their services, especially the ultra fast fragments service of which I have yet to get a faulty clone from!"
Status:
ReviewerMember since: 2021
Organization: USZ
Simply an amazing service!
Application Area:Expression of gene of interest in B cells using plasmids
"My life completely changed when I discovered Twist Bioscience because I started to buy the plasmids that I need instead of doing them by myself. The results are much faster, reproducible and reliable. The online platform is super easy to use and the team of customer service is available for you at any time in less than one minute of waiting. The service is amazing! Thank you!"
Status:
ReviewerMember since: 2021
Organization: UT Southwestern Medical Center
It's a great service and provided me with great synthesized gene in a plasmid.
Application Area:Gene synthesis
"The synthesized gene in a plasmid is of good quality. I was able to easily clone the gene into different vectors for next steps."
Status:
ReviewerMember since: 2021
Organization: Amyris
Twist gene synthesis greatly improve my productivity.
Application Area:Synthetic biology
"Twist clonal genes have high quality and ordering interface is very easy to use."
Status:
ReviewerMember since: 2021
Organization: CNRS
The best service on the market.
Application Area:Post-translational modifications role on DNA replication
"It is really easy to upload sequences, the people from the "helpdesk" are really efficient and very helpful. The product was delivered as expected. The price is ridiculously low."
Status:
ReviewerMember since: 2021
Organization: HudsonAlpha Institute for Biotechnology
Efficient gene synthesis and cloning.
Application Area:Gene structure-function analysis
"Gene synthesis antiquates some tedious aspects of experimentation. Twist Biosciences streamlines the process of ordering expression clones. All steps up to and including quote generation are handled through their intuitive web-based ordering interface. The entire process is simple and efficient, and allows you to keep track of multiple projects and to share information with collaborators. Twist follows up after the ordering process. I ordered a set of gene variants cloned into expression vectors and I received them within 2 weeks. The price was half that of competitors ($0.09/base). I will continue to use Twist for future gene synthesis services."
Status:
ReviewerMember since: 2020
Organization: Biochemize
Definitely my favorite gene supplier.
Application Area:Genes for E. coli protein expression
"Easy to order, very intuitive. Automatic quotation price."
Status:
ReviewerMember since: 2020
Organization: University of Helsinki
Very good service.
Application Area:Gene synthesis and cloning for protein production
"Super easy to order. The whole process is very smoothly designed. Very good time estimates for the whole process. Excellent communication."
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base. Twist’s Clonal Genes offering gives you the flexibility to get the DNA you want, the way you want it. Choose the amount of DNA, and the format you want it in to drive your research. Because every clonal gene is NGS-verified, you can be sure you’re getting the perfect cloned gene every time.
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