Multiplexed Gene Fragments

Twist Multiplexed Gene Fragments are double stranded gene fragment sequences of up to 500 base pairs in length that are delivered as a pool instead of one fragment per well matrix. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.
Multiplexed Gene Fragments are synthesized from user defined sequences in lengths of up to 500 base pairs per gene. These user-defined sequences are initially synthesized into oligo pools of up to 500 nucleotides in length and are then amplified in double-stranded DNA, and delivered as multiplexed gene fragments that have been carefully analyzed to ensure >90% of genes in the pool are the correct length. This product is optimized for rapid, high-throughput screening applications.