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Oligo Pools

Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run.

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Twist Bioscience

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Average Rating 5.0

|5Scientists have reviewed this product

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Oligo pool with excellent quality that I highly recommend


Average Rating 5.0

Application Area:

Deep mutational scanning of protein degrons

I ordered a 68bp oligo pool with >5000 sequences for deep mutational scanning of protein degrons. The pool had good yield and was easy to amplify. The pool was also very uniform. More than 96% of the desired sequences could be detected after all data analysis steps. Error rate is low. I could rarely see sequences that were not included in the original oligo pool design.

Review Date: 4 Nov 2022 | Twist Bioscience

Good service with great product on oligopool synthesis.


Average Rating 5.0

Application Area:


Easy platform to use and order complex oligopool for downstream FISH probe generation. So far, every oligopool have been working and yield is reasonable. The fast turnaroud time is important for scientific work and it is also very easy to talk to their customer service support for anything regarding the order.

Review Date: 20 Nov 2021 | Twist Bioscience

Best in class low error rates and cheap pricing.


Average Rating 5.0

Application Area:

Synthesis of barcoded DNA fragments for cloning

TWIST's platform is super simple, their DNA synthesis tech has best-in-class error rates, and cheapest pricing. Turnaround time is sometimes a day or two slower than competitors, but as the error rate is so low we save time overall.

Review Date: 21 Mar 2021 | Twist Bioscience

Uniform oligo pool


Average Rating 5.0

Application Area:

Library for peptide screening

We ordered the 150mer oligo pool coding for a peptide library, to be used in a binding screen. The oligo pool was highly uniform, which turned out to be very valuable for our screening pipeline. We were able to avoid over and underrepresented peptides, and retrieve ~97% designed peptides in our screen.

Review Date: 16 Dec 2020 | Twist Bioscience

High quality, wouldn't have gotten my PhD without them!


Average Rating 5.0

Application Area:

Test thousands of different genetic designs at once

Twist Oligo Pools are a great way to test hundreds to thousands of different genetic designs at once. Great diversity and quality of oligos.

Review Date: 27 Jan 2020 | Twist Bioscience

Oligo pools are utilized in many applications including generation of CRISPR guide RNA (sgRNA) libraries, peptide screens, protein screens, and high-throughput reporter assays. Accuracy and uniformity of oligo synthesis are critical to ensure high quality oligo libraries are built and screens are precisely targeted. Our industry-leading low synthesis error rate and uniform sequence representation result in high quality custom oligo libraries that enable the most efficient screens.


  • Oligo Length: Up to 300 nt
  • Oligo Pool Size: Minimum of 2,000 sequences with no maximum
  • Yield>0.2 fmol for each oligo

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