Member since: 2016
Organization: University of North Carolina at Charlotte
A very handy and excellent results reproducing machine
Application Area: Excellent Quantifying Instrument
"This machine is excellent for gene expression analysis. Little complicated to learn the software and options initially but once you learn the points it is easy to use and provide reliable and great results. The 96 well plate can run in one go and the digital and touch screen is very helpful. Highly recommended for the gene expression and quantifying work."
Use the CFX96 Deep Well Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Deep Well Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.
The CFX96 Deep Well Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).
The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR® Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.
The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.
The following modules can be used with the C1000 Touch Thermal Cycler Chassis:
*Starter package contains SsoAdvanced™ Universal SYBR® Green Supermix ( 1725270 ), Microseal® 'B' Adhesive Seals ( MSB1001 ), and Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 ) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ( HSP3805 ).
The CFX96 Deep Well Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software control. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.
Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II , our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.