Supel™ Tox SPE Cartridges
Fast and Simple Cleanup for Mycotoxin Analysis.The need for a quick, simplistic sample cleanup approach prior to chromatographic mycotoxin analysis has brought about SPE cartridges that significantly decrease sample prep time, increase reproducibility, and are more user friendly as compared to the industry standard immunoaffinity columns. In addition the Supel Tox SPE approach requires less equipment and fewer consumables.Unli…
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Fast and Simple Cleanup for Mycotoxin Analysis.
The need for a quick, simplistic sample cleanup approach prior to chromatographic mycotoxin analysis has brought about SPE cartridges that significantly decrease sample prep time, increase reproducibility, and are more user friendly as compared to the industry standard immunoaffinity columns. In addition the Supel Tox SPE approach requires less equipment and fewer consumables.
Unlike the multiple step "bind and elute" strategy implemented when using immunoaffinity columns, the Supel Tox AflaZea, DON, and Tricho SPE cartridges employ an "interference removal" strategy which saves time by eliminating wash steps prior to elution of aflatoxin, zearalenone, deoxynivalenol, and tricothecenes (type A and B), respectively. Cartridges removing interferences associated with analysis of the above and fumonisins (B1 and B2) as well as ochratoxin A are also available as a part of our Supel Tox product offering.
Features and Benefits:
• Better reproducibility than the current industry standard, immunoaffinity columns
• Removes interferences associated with mycotoxin analysis
• Basic and quick methodology, requires little additional method development
• Sample preparation time is ten times faster than when using immunoaffinity columns
• Improved shelf life over immunoaffinity columns due to thermally stable format
• No refrigeration required for shipping and storage
Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk using SPE Cleanup and LC/MS Analysis
Aflatoxins are produced by fungi and these species can infect important cereal grains, including maize, as well as ground (peanuts) and tree nuts. The focus of this study is the metabolite of AFB1, aflatoxin M1 (AFM1) that can be present in the milk of lactating dairy animals that have consumed aflatoxin contaminated feeds. A fast method for cleanup and analysis has been developed using Supel™ Tox AflaZea SPE, Ascentis® Express C18 HPLC column and LC/MS.
Simplify and Reduce Variability During SPE Cleanup of Wheat Extracts Prior to the HPLC Analysis of the Mycotoxin Deoxynivalenol
In this application note, the extraction and cleanup method of the Supel™ Tox DON SPE cartridge was compared to that of a commercially available IAC column in order to determine differences pertaining to ease of use and process time when cleaning wheat matrix samples.
Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste
This application note compares aflatoxin sample purification methods utilizing IAC and SPE cleanup methods. Sample processing time, product performance, and process simplicity are evaluated.
