SpectraMax DuoLuc Reporter Assay Kit
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by normalizing reporter expression to a control Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors…
Place your order directly with the manufacturer.
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by normalizing reporter expression to a control Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors Simplified data acquisition and analysis with preconfigured protocol in SoftMax® Pro Software Support for your complete solution of reader, software, and assay
Optimized plate reader assays for breakthrough drug discovery research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers. However, assay optimization remains an essential and often time-consuming step to ensure the desired sensitivity and specificity to the target of interest. Molecular Devices presents its range of easy-to-use, robust assay kits for protein, DNA, and cell-based assays. Discover how these assay kits are optimized for the highest data quality on Molecular Devices instruments, including compatible software to streamline your workflow.
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions, with Luciferases being the most common of these.
In this application note, Molecular Devices demonstrates how the DuoLuc reporter assay and SpectraMax iD5 reader can be utilized to detect nuclear factor-κB (NF-κB) activation in mammalian cell models.
Detect Dual Luciferase Expression on the FlexStation 3 Microplate Reader
Reporter gene assays are important tools for studying gene expression associated with the activation of cellular pathways. Cells are transfected with a plasmid containing the reporter gene and a sequence of interest, typically a promoter or other transcriptional control element. When the promoter is activated, the reporter gene is expressed and its levels can be measured.
