Gemini EM Microplate Reader
Re-invent your fluorescence assays
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Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets you determine the optimal excitation and emission settings for your creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them for you. Switch easily between top and bottom reading modes for solution- and cell-based assays.
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However for biological applications involving small samples, such as next-generation sequencing and quantitation of DNA amplification products, sensitive methods are needed.
In this application note, Molecular Devices demonstrates how the Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA.
