ReadyToProcess™ prepacked chromatography columns
Validated high performance prepacked columns for scale-up and GMP biomanufacturing
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Cytiva HisTrap columns are reliable, easy to use, and consistently deliver high purity His tagged protein purification
Protein Purification
We have been using Cytiva HisTrap columns regularly for His-tagged protein purification, and they have been consistently reliable. The columns are straightforward to set up and work smoothly with both ÄKTA systems and manual workflows, which makes them easy to fit into day-to-day lab routines. Binding to the Ni Sepharose resin is strong and specific, even when working with complex samples. We usually see clean, sharp elution peaks and good purity of the target protein without needing extensive optimization. Backpressure remains low, and the performance stays consistent across multiple runs. A big plus is that the columns come prepacked and ready to use, saving time and reducing variability between experiments. They also hold up well to regeneration and reuse, which makes them practical and cost effective for routine purification work. Overall, Cytiva HisTrap columns are dependable, user friendly, and deliver consistent results, making them a solid choice for both exploratory and routine protein purification.
Review Date: 29 Jan 2026 | Cytiva
ReadytoProcess™ columns have a robust design and validated column packing methods for consistent performance
Features:
- Standardized format for reduced costs and short delivery lead times.
- Reduced costs by eliminating the need for column packing, preparation, and validation procedures.
- Designed to work with ÄKTA ready single-use chromatography systems and UNICORN™ control software.
- Available for Capto™ and MabSelect™ resin families, in sizes from 80 to 600 mm inner diameter and standard bed heights of 15, 20 and 25 cm. Additional bed heights are available upon request.
Extractables in single-use systems used in ADC manufacturing
Antibody-drug conjugates (ADCs) are biotherapeutic molecules consisting of a cytotoxin coupled to a monoclonal antibody (mAb) by a linker. Single-use (SU) systems are well suited to ADC manufacturing for several reasons, such as minimizing operator exposure to toxins. Cytiva supports the adoption of SU technologies in ADC production by extractables studies, with conditions relevant to current ADC manufacturing processes. Discover how three SU products: ReadyToProcess™ 1 L column housing, disposable ÄKTA ready™ low flow kit for chromatographic systems, and ÄKTA readyflux™ flow kit for tangential flow filtration, demonstrate low levels of extractables with organic solvents DMSO and DMA.
